Počet záznamů: 1
Insect-Symbiont Gene Expression in the Midgut Bacteriocytes of a Blood-Sucking Parasite
- 1.
SYSNO ASEP 0538246 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Insect-Symbiont Gene Expression in the Midgut Bacteriocytes of a Blood-Sucking Parasite Tvůrce(i) Husník, Filip (BC-A) ORCID, RID
Hypša, Václav (BC-A) RID, ORCID
Darby, A. C. (GB)Celkový počet autorů 3 Zdroj.dok. Genome Biology and Evolution. - : Oxford University Press - ISSN 1759-6653
Roč. 12, č. 4 (2020), s. 429-442Poč.str. 14 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova mutualist wigglesworthia ; host-cell ; pgrp-lb ; genome ; aphid ; louse ; endosymbionts ; evolution ; protein ; sodalis ; RNA-Seq ; B-vitamins ; parasites ; symbiotic bacteria ; interactions ; zinc ; immunity Vědní obor RIV EA - Morfologické obory a cytologie Obor OECD Genetics and heredity (medical genetics to be 3) Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000538703000016 EID SCOPUS 85084272550 DOI https://doi.org/10.1093/gbe/evaa032 Anotace Animals interact with a diverse array of both beneficial and detrimental microorganisms. In insects, these symbioses in many cases allow feeding on nutritionally unbalanced diets. It is, however, still not clear how are obligate symbioses maintained at the cellular level for up to several hundred million years. Exact mechanisms driving host-symbiont interactions are only understood for a handful of model species and data on blood-feeding hosts with intracellular bacteria are particularly scarce. Here, we analyzed interactions between an obligately blood-sucking parasite of sheep, the louse fly Melophagus ovinus, and its obligate endosymbiont, Arsenophonus melophagi. We assembled a reference transcriptome for the insect host and used dual RNA-Seq with five biological replicates to compare expression in the midgut cells specialized for housing symbiotic bacteria (bacteriocytes) to the rest of the gut (foregut-hindgut). We found strong evidence for the importance of zinc in the system likely caused by symbionts using zinc-dependent proteases when acquiring amino acids, and for different immunity mechanisms controlling the symbionts than in closely related tsetse flies. Our results show that cellular and nutritional interactions between this blood-sucking insect and its symbionts are less intimate than what was previously found in most plant-sap sucking insects. This finding is likely interconnected to several features observed in symbionts in blood-sucking arthropods, particularly their midgut intracellular localization, intracytoplasmic presence, less severe genome reduction, and relatively recent associations caused by frequent evolutionary losses and replacements. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2021 Elektronická adresa https://academic.oup.com/gbe/article/12/4/429/5739960
Počet záznamů: 1