Počet záznamů: 1
A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response
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SYSNO ASEP 0536914 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response Tvůrce(i) Dumková, J. (CZ)
Smutná, Tereza (UZFG-Y) ORCID
Vrlíková, Lucie (UZFG-Y) ORCID
Dočekal, Bohumil (UIACH-O) RID, ORCID
Kristeková, Daniela (UZFG-Y) ORCID
Večeřa, Zbyněk (UIACH-O) RID
Husáková, Z. (CZ)
Jakešová, Veronika (UZFG-Y)
Jedličková, Adriena (UZFG-Y) ORCID
Mikuška, Pavel (UIACH-O) RID, ORCID
Alexa, Lukáš (UIACH-O) ORCID, RID
Coufalík, Pavel (UIACH-O) ORCID, RID
Tvrdoňová, M. (CZ)
Křůmal, Kamil (UIACH-O) RID, ORCID
Vaculovič, T. (CZ)
Kanický, V. (CZ)
Hampl, A. (CZ)
Buchtová, Marcela (UZFG-Y) RID, ORCIDČíslo článku 8738 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 22 (2020)Poč.str. 27 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova clearance ; lead nanoparticles ; inhalation ; LA-ICP-MS imaging ; toxicity Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology Vědní obor RIV – spolupráce Ústav analytické chemie - Analytická chemie, separace CEP GA20-02203S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 ; UIACH-O - RVO:68081715 UT WOS 000594717000001 EID SCOPUS 85096315776 DOI 10.3390/ijms21228738 Anotace The inhalation of metal (including lead) nanoparticles poses a real health issue to people and animals living in polluted and/or industrial areas. In this study, we exposed mice to lead(II) nitrate nanoparticles [Pb(NO3)(2) NPs], which represent a highly soluble form of lead, by inhalation. We aimed to uncover the effects of their exposure on individual target organs and to reveal potential variability in the lead clearance. We examined (i) lead biodistribution in target organs using laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) and atomic absorption spectrometry (AAS), (ii) lead effect on histopathological changes and immune cells response in secondary target organs and (iii) the clearance ability of target organs. In the lungs and liver, Pb(NO3)(2) NP inhalation induced serious structural changes and their damage was present even after a 5-week clearance period despite the lead having been almost completely eliminated from the tissues. The numbers of macrophages significantly decreased after 11-week Pb(NO3)(2) NP inhalation, conversely, abundance of alpha-smooth muscle actin (alpha-SMA)-positive cells, which are responsible for augmented collagen production, increased in both tissues. Moreover, the expression of nuclear factor kappa B (NF-kappa B) and selected cytokines, such as tumor necrosis factor alpha (TNF alpha), transforming growth factor beta 1 (TGF beta 1), interleukin 6(IL-6), IL-1 alpha and IL-1 beta , displayed a tissue-specific response to lead exposure. In summary, diminished inflammatory response in tissues after Pb(NO3)(2) NPs inhalation was associated with prolonged negative effect of lead on tissues, as demonstrated by sustained pathological changes in target organs, even after long clearance period. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2021 Elektronická adresa https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=820955896
Počet záznamů: 1