Počet záznamů: 1
DNA damage triggers reprogramming of differentiated cells into stem cells in Physcomitrella
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SYSNO ASEP 0532864 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název DNA damage triggers reprogramming of differentiated cells into stem cells in Physcomitrella Tvůrce(i) Gu, N. (CN)
Tamada, Y. (JP)
Imai, A. (JP)
Palfalvi, G. (JP)
Kabeya, Y. (JP)
Shigenobu, S. (JP)
Ishikawa, M. (JP)
Angelis, Karel (UEB-Q) RID, ORCID
Chen, C. (CN)
Hasebe, M. (JP)Celkový počet autorů 10 Zdroj.dok. Nature Plants - ISSN 2055-026X
Roč. 6, č. 9 (2020), s. 1098-1105Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova 2-TAILED COMET ASSAY ; MOSS ; REGENERATION Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP LTC17047 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000560891700003 EID SCOPUS 85089512572 DOI 10.1038/s41477-020-0745-9 Anotace DNA damage can result from intrinsic cellular processes and from exposure to stressful environments. Such DNA damage generally threatens genome integrity and cell viability1. However, here we report that the transient induction of DNA strand breaks (single-strand breaks, double-strand breaks or both) in the moss Physcomitrella patens can trigger the reprogramming of differentiated leaf cells into stem cells without cell death. After intact leafy shoots (gametophores) were exposed to zeocin, an inducer of DNA strand breaks, the STEMCELL-INDUCING FACTOR 1 (STEMIN1)2 promoter was activated in some leaf cells. These cells subsequently initiated tip growth and underwent asymmetric cell divisions to form chloronema apical stem cells, which are in an earlier phase of the life cycle than leaf cells and have the ability to form new gametophores. This DNA-strand-break-induced reprogramming required the DNA damage sensor ATR kinase, but not ATM kinase, together with STEMIN1 and closely related proteins. ATR was also indispensable for the induction of STEMIN1 by DNA strand breaks. Our findings indicate that DNA strand breaks, which are usually considered to pose a severe threat to cells, trigger cellular reprogramming towards stem cells via the activity of ATR and STEMINs. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2021 Elektronická adresa http://doi.org/10.1038/s41477-020-0745-9
Počet záznamů: 1