Počet záznamů: 1
Novel versatile 3D bio-scaffold made of natural biocompatible hagfish exudate for tissue growth and organoid modeling
- 1.
SYSNO ASEP 0532432 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Novel versatile 3D bio-scaffold made of natural biocompatible hagfish exudate for tissue growth and organoid modeling Tvůrce(i) Dastjerdi, M.B. (JP)
Amini, A. (AU)
Nazari, M. (KW)
Cheng, Ch. (CN)
Benson, Veronika (MBU-M) RID, ORCID
Gholami, A. (IR)
Ghasemi, Y. (IR)Zdroj.dok. International Journal of Biological Macromolecules. - : Elsevier - ISSN 0141-8130
Roč. 158, SEP (2020), s. 894-902Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova 3-dimensional cell-culture ; hydrogel scaffolds ; aqueous-solution ; removal Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000564487500004 EID SCOPUS 85084505762 DOI 10.1016/j.ijbiomac.2020.05.024 Anotace Hagfish exudate is a natural biological macromolecule made of keratin intermediate filament protein skeins and mucin vesicles. Here, we successfully examined this remarkable biomaterial as a substrate for three-dimensional (3D) cell culturing purposes. After the sterilization with chloroform vapor, Dulbecco's modified eagle medium was mixed with the exudate to rupture the vesicles and skeins, a highly soft, adherent, fibrous and biocompatible hydrogel was formed. A variety of cells, including Hela-FUCCI, NMuMG-FUCCI, 10T1/2 and C2C12, was cultured on the hagfish exudate. A remarkable 3D growth by-2.5 folds after day 3,-5 folds after day 5,-10 folds after day 7 and15 folds after day 14 were seen compared to day one of culturing in the hagfish exudate scaffold. In addition, the phase contrast, fluorescent and confocal microscopy observations confirmed the organoid shape formation within the three-week culture. The viability of cells was almost 100% indicating the great in vitro and in vivo potential of this exceptional biomaterial with no cytotoxic effect. (c) 2020 Elsevier B.V. All rights reserved. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0141813020331718?via%3Dihub
Počet záznamů: 1