Počet záznamů: 1
gamma-Tubulin interacts with E2F transcription factors to regulate proliferation and endocycling in Arabidopsis
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SYSNO ASEP 0524624 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název gamma-Tubulin interacts with E2F transcription factors to regulate proliferation and endocycling in Arabidopsis Tvůrce(i) Kallai, B.M. (HU)
Kourová, Hana (MBU-M)
Chumová, Jana (MBU-M)
Papdi, C. (GB)
Trögelová, Lucie (MBU-M) ORCID
Kofroňová, Olga (MBU-M) RID, ORCID
Hozák, Pavel (UMG-J) RID, ORCID
Filimonenko, Vlada (UMG-J) RID, ORCID
Mészáros, T. (HU)
Magyar, Z. (HU)
Börge, L. (GB)
Binarová, Pavla (MBU-M) RID, ORCIDZdroj.dok. Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 71, č. 4 (2020), s. 1265-1277Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Arabidopsis ; endoreduplication ; E2F transcription factors Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Cell biology Vědní obor RIV – spolupráce Ústav molekulární genetiky CEP GA15-11657S GA ČR - Grantová agentura ČR LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_013/0001775 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; UMG-J - RVO:68378050 UT WOS 000518530400009 EID SCOPUS 85080840986 DOI 10.1093/jxb/erz498 Anotace gamma-Tubulin is associated with microtubule nucleation, but evidence is accumulating in eukaryotes that it also functions in nuclear processes and in cell division control independently of its canonical role. We found that in Arabidopsis thaliana, gamma-tubulin interacts specifically with E2FA, E2FB, and E2FC transcription factors both in vitro and in vivo. The interaction of gamma-tubulin with the E2Fs is not reduced in the presence of their dimerization partners (DPs) and, in agreement, we found that gamma-tubulin interaction with E2Fs does not require the dimerization domain. gamma-Tubulin associates with the promoters of E2F-regulated cell cycle genes in an E2F-dependent manner, probably in complex with the E2F-DP heterodimer. The up-regulation of E2F target genes PCNA, ORC2, CDKB1,1, and CCS52A under gamma-tubulin silencing suggests a repressive function for gamma-tubulin at G(1)/S and G(2)/M transitions, and the endocycle, which is consistent with an excess of cell division in some cells and enhanced endoreduplication in others in the shoot and young leaves of gamma-tubulin RNAi plants. Altogether, our data show ternary interaction of gamma-tubulin with the E2F-DP heterodimer and suggest a repressive role for gamma-tubulin with E2Fs in controlling mitotic activity and endoreduplication during plant development. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://academic.oup.com/jxb/article/71/4/1265/5613973
Počet záznamů: 1