Počet záznamů: 1
The beta subunit of nascent polypeptide associated complex plays a role in flowers and siliques development of arabidopsis thaliana
- 1.
SYSNO ASEP 0524246 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The beta subunit of nascent polypeptide associated complex plays a role in flowers and siliques development of arabidopsis thaliana Tvůrce(i) Fíla, Jan (UEB-Q) RID, ORCID
Klodová, Božena (UEB-Q) ORCID
Potěšil, D. (CZ)
Juříček, Miloslav (UEB-Q) RID, ORCID
Šesták, Petr (UEB-Q) ORCID
Zdráhal, Z. (CZ)
Honys, David (UEB-Q) RID, ORCIDCelkový počet autorů 7 Číslo článku 2065 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 6 (2020)Poč.str. 31 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Arabidopsis thaliana ; Chaperone ; Flower bud proteome ; Flower bud transcriptome ; Male gametophyte ; Nascent polypeptide-associated complex Vědní obor RIV EF - Botanika Obor OECD Plant sciences, botany CEP LTC18043 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA18-02448S GA ČR - Grantová agentura ČR GA19-01723S GA ČR - Grantová agentura ČR EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000529890200161 EID SCOPUS 85082085747 DOI 10.3390/ijms21062065 Anotace The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, α and β, and was shown to bind to the nascent polypeptides newly emerging from the ribosomes. NAC function was widely described in yeast and several information are also available about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes encoding NACα subunit, and two genes encoding NACβ. Double homozygous mutant in both genes coding for NACβ was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Both NACβ genes were characterized in more detail—the phenotype of the double homozygous mutant was complemented by a functional NACβ copy. Then, both NACβ genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nacβ mutation, the flower buds’ transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NACβ subunits in stress responses, male gametophyte development, and photosynthesis. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2021 Elektronická adresa http://doi.org/10.3390/ijms21062065
Počet záznamů: 1