Počet záznamů: 1
Spatial and Temporal Variability of Plant Leaf Responses Cascade after PSII Inhibition: Raman, Chlorophyll Fluorescence and Infrared Thermal Imaging
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SYSNO ASEP 0523912 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Spatial and Temporal Variability of Plant Leaf Responses Cascade after PSII Inhibition: Raman, Chlorophyll Fluorescence and Infrared Thermal Imaging Tvůrce(i) Vítek, Petr (UEK-B) RID, SAI
Veselá, Barbora (UEK-B) SAI, RID
Klem, Karel (UEK-B) RID, ORCID, SAICelkový počet autorů 3 Číslo článku 1015 Zdroj.dok. Sensors. - : MDPI
Roč. 20, č. 4 (2020)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova carotenoid oxidation-products ; photosystem-ii ; xanthophyll cycle ; singlet oxygen ; abscisic-acid ; ft-raman ; stomatal conductance ; stress signals ; nitric-oxide ; spectroscopy ; Raman mapping ; carotenoids ; zeaxanthin ; photosynthesis ; oxidative stress ; xanthophyll cycle ; photoinhibition Vědní obor RIV EF - Botanika Obor OECD Plant sciences, botany CEP LTC18036 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CzeCOS III - 90123 - Ústav výzkumu globální změny AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora UEK-B - RVO:86652079 UT WOS 000522448600066 EID SCOPUS 85079572248 DOI 10.3390/s20041015 Anotace The use of photosystem II (PSII) inhibitors allows simulating cascade of defense and damage responses, including the oxidative stress. In our study, PSII inhibiting herbicide metribuzin was applied to the leaf of the model plant species Chenopodium album. The temporally and spatially resolved cascade of defense responses was studied noninvasively at the leaf level by combining three imaging approaches: Raman spectroscopy as a principal method, corroborated by chlorophyll a fluorescence (ChlF) and infrared thermal imaging. ChlF imaging show time-dependent transport in acropetal direction through veins and increase of area affected by metribuzin and demonstrated the ability to distinguish between fast processes at the level of electron transport (1 V-j) from slow processes at the level of non-photochemical energy dissipation (NPQ) or maximum efficiency of PSII photochemistry (Fv/Fm). The high-resolution resonance Raman images show zones of local increase of carotenoid signal 72 h after the herbicide application, surrounding the damaged tissue, which points to the activation of defense mechanisms. The shift in the carotenoid band indicates structural changes in carotenoids. Finally, the increase of leaf temperature in the region surrounding the spot of herbicide application and expanding in the direction to the leaf tip proves the metribuzin effect on slow stomata closure. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/1424-8220/20/4/1015
Počet záznamů: 1