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LmxM.22.0250-Encoded Dual Specificity Protein/Lipid Phosphatase Impairs Leishmania mexicana Virulence In Vitro
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SYSNO ASEP 0522703 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název LmxM.22.0250-Encoded Dual Specificity Protein/Lipid Phosphatase Impairs Leishmania mexicana Virulence In Vitro Tvůrce(i) Kraeva, N. (CZ)
Leštinová, T. (CZ)
Ishemgulova, A. (CZ)
Majerová, K. (CZ)
Butenko, Anzhelika (BC-A) RID, ORCID
Vaselek, S. (RS)
Bespyatykh, Y. (RU)
Charyyeva, A. (CZ)
Spitzova, T. (CZ)
Kostygov, A.Y. (CZ)
Lukeš, Julius (BC-A) RID, ORCID
Volf, P. (CZ)
Votýpka, Jan (BC-A) RID, ORCID
Yurchenko, V. (CZ)Celkový počet autorů 14 Číslo článku 241 Zdroj.dok. Pathogens. - : MDPI
Roč. 8, č. 4 (2019)Poč.str. 14 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova mycobacterium-tuberculosis ; tyrosine phosphatases ; expression system ; mptpb ; identification ; insights ; kinases ; genome ; LmDUSP1 ; virulence factor ; Leishmania infection Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP LL1601 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_019/0000759 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000506652300084 EID SCOPUS 85075433449 DOI 10.3390/pathogens8040241 Anotace Protein phosphorylation/dephosphorylation is an important regulatory mechanism that controls many key physiological processes. Numerous pathogens successfully use kinases and phosphatases to internalize, replicate, and survive, modifying the host ' s phosphorylation profile or signal transduction pathways. Multiple phosphatases and kinases from diverse bacterial pathogens have been implicated in human infections before. In this work, we have identified and characterized the dual specificity protein/lipid phosphatase LmDUSP1 as a novel virulence factor governing Leishmania mexicana infection. The LmDUSP1-encoding gene (LmxM.22.0250 in L. mexicana) has been acquired from bacteria via horizontal gene transfer. Importantly, its orthologues have been associated with virulence in several bacterial species, such as Mycobacterium tuberculosis and Listeria monocytogenes. Leishmania mexicana with ablated LmxM.22.0250 demonstrated severely attenuated virulence in the experimental infection of primary mouse macrophages, suggesting that this gene facilitates Leishmania pathogenicity in vertebrates. Despite significant upregulation of LmxM.22.0250 expression in metacyclic promastigotes, its ablation did not affect the ability of mutant cells to differentiate into virulent stages in insects. It remains to be further investigated which specific biochemical pathways involve LmDUSP1 and how this facilitates the parasite ' s survival in the host. One of the interesting possibilities is that LmDUSP1 may target host ' s substrate(s), thereby affecting its signal transduction pathways. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2020 Elektronická adresa https://www.mdpi.com/2076-0817/8/4/241
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