Počet záznamů: 1
Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF in Immunity, Suggesting That Their Interacting Adipokine Role Has Been Acquired Only in Mammals
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SYSNO ASEP 0520648 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF in Immunity, Suggesting That Their Interacting Adipokine Role Has Been Acquired Only in Mammals Tvůrce(i) Seroussi, E. (IL)
Knytl, M. (CZ)
Pitel, F. (FR)
Elleder, Daniel (UMG-J) RID
Krylov, V. (CZ)
Leroux, S. (FR)
Morisson, M. (FR)
Yosefi, S. (IL)
Miyara, S. (IL)
Ganesan, S. (IL)
Ruzal, M. (IL)
Andersson, L. (SE)
Friedman-Einat, M. (IL)Celkový počet autorů 13 Číslo článku 4489 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 20, č. 18 (2019)Poč.str. 15 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova radiation-hybrid mapping ; fish-tsa ; chicken ; tnf ; immune system ; leptin ; digestive tract ; duodenum Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Immunology CEP LO1419 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 000489100500168 DOI 10.3390/ijms20184489 Anotace In mammals, leptin and tumor-necrosis factor (TNF) are prominent interacting adipokines mediating appetite control and insulin sensitivity. While TNF pleiotropically functions in immune defense and cell survival, leptin is largely confined to signaling energy stores in adipocytes. Knowledge about the function of avian leptin and TNF is limited and they are absent or lowly expressed in adipose, respectively. Employing radiation-hybrid mapping and FISH-TSA, we mapped TNF and its syntenic genes to chicken chromosome 16 within the major histocompatibility complex (MHC) region. This mapping position suggests that avian TNF has a role in regulating immune response. To test its possible interaction with leptin within the immune system and beyond, we compared the transcription patterns of TNF, leptin and their cognate receptors obtained by meta-analysis of GenBank RNA-seq data. While expression of leptin and its receptor (LEPR) were detected in the brain and digestive tract, TNF and its receptor mRNAs were primarily found in viral-infected and LPS-treated leukocytes. We confirmed leptin expression in the duodenum by immunohistochemistry staining. Altogether, we suggest that whereas leptin and TNF interact as adipokines in mammals, in birds, they have distinct roles. Thus, the interaction between leptin and TNF may be unique to mammals. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2020 Elektronická adresa https://www.mdpi.com/1422-0067/20/18/4489
Počet záznamů: 1