Počet záznamů: 1
Pigment-protein complexes are organized into stable microdomains in cyanobacterial thylakoids
- 1.
SYSNO ASEP 0519560 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Pigment-protein complexes are organized into stable microdomains in cyanobacterial thylakoids Tvůrce(i) Strašková, Adéla (MBU-M)
Steinbach, Gabor (MBU-M)
Konert, Grzegorz (MBU-M) ORCID
Kotabová, Eva (MBU-M) RID, ORCID
Komenda, Josef (MBU-M) RID, ORCID
Tichý, Martin (MBU-M) RID
Kaňa, Radek (MBU-M) RID, ORCIDČíslo článku 148053 Zdroj.dok. Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728
Roč. 1860, č. 12 (2019)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Photosynthesis ; Confocal microscopy ; Thylakoid membrane Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA16-10088S GA ČR - Grantová agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED2.1.00/19.0392 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000504950800001 EID SCOPUS 85072072358 DOI 10.1016/j.bbabio.2019.07.008 Anotace Thylakoids are the place of the light-photosynthetic reactions. To gain maximal efficiency, these reactions are conditional to proper pigment-pigment and protein-protein interactions. In higher plants thylakoids, the interactions lead to a lateral asymmetry in localization of protein complexes (i.e. granal/stromal thylakoids) that have been defined as a domain-like structures characteristic by different biochemical composition and function (Albertsson P-A. 2001,Trends Plant Science 6: 349-354). We explored this complex organization of thylakoid pigment-proteins at single cell level in the cyanobacterium Synechocysas sp. PCC 6803. Our 3D confocal images captured heterogeneous distribution of all main photosynthetic pigment-protein complexes (PPCs), Photosystem I (fluorescently tagged by YFP), Photosystem II and Phycobilisomes. The acquired images depicted cyanobacterial thylakoid membrane as a stable, mosaic-like structure formed by microdomains (MDs). These microcompartments are of sub-micrometer in sizes (similar to 0.5-1.5 mu m), typical by particular PPCs ratios and importantly without full segregation of observed complexes. The most prevailing MD is represented by MD with high Photosystem I content which allows also partial separation of Photosystems like in higher plants thylakoids. We assume that MDs stability (in minutes) provides optimal conditions for efficient excitation/electron transfer. The cyanobacterial MDs thus define thylakoid membrane organization as a system controlled by co-localization of three main PPCs leading to formation of thylakoid membrane mosaic. This organization might represent evolutional and functional precursor for the granal/stromal spatial heterogeneity in photosystems that is typical for higher plant thylakoids. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0005272819300908?via%3Dihub
Počet záznamů: 1