Počet záznamů: 1  

Pigment-protein complexes are organized into stable microdomains in cyanobacterial thylakoids

  1. 1.
    SYSNO ASEP0519560
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevPigment-protein complexes are organized into stable microdomains in cyanobacterial thylakoids
    Tvůrce(i) Strašková, Adéla (MBU-M)
    Steinbach, Gabor (MBU-M)
    Konert, Grzegorz (MBU-M) ORCID
    Kotabová, Eva (MBU-M) RID, ORCID
    Komenda, Josef (MBU-M) RID, ORCID
    Tichý, Martin (MBU-M) RID
    Kaňa, Radek (MBU-M) RID, ORCID
    Číslo článku148053
    Zdroj.dok.Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728
    Roč. 1860, č. 12 (2019)
    Poč.str.15 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovaPhotosynthesis ; Confocal microscopy ; Thylakoid membrane
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    CEPGA16-10088S GA ČR - Grantová agentura ČR
    LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    ED2.1.00/19.0392 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOmezený přístup
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000504950800001
    EID SCOPUS85072072358
    DOI10.1016/j.bbabio.2019.07.008
    AnotaceThylakoids are the place of the light-photosynthetic reactions. To gain maximal efficiency, these reactions are conditional to proper pigment-pigment and protein-protein interactions. In higher plants thylakoids, the interactions lead to a lateral asymmetry in localization of protein complexes (i.e. granal/stromal thylakoids) that have been defined as a domain-like structures characteristic by different biochemical composition and function (Albertsson P-A. 2001,Trends Plant Science 6: 349-354). We explored this complex organization of thylakoid pigment-proteins at single cell level in the cyanobacterium Synechocysas sp. PCC 6803. Our 3D confocal images captured heterogeneous distribution of all main photosynthetic pigment-protein complexes (PPCs), Photosystem I (fluorescently tagged by YFP), Photosystem II and Phycobilisomes. The acquired images depicted cyanobacterial thylakoid membrane as a stable, mosaic-like structure formed by microdomains (MDs). These microcompartments are of sub-micrometer in sizes (similar to 0.5-1.5 mu m), typical by particular PPCs ratios and importantly without full segregation of observed complexes. The most prevailing MD is represented by MD with high Photosystem I content which allows also partial separation of Photosystems like in higher plants thylakoids. We assume that MDs stability (in minutes) provides optimal conditions for efficient excitation/electron transfer. The cyanobacterial MDs thus define thylakoid membrane organization as a system controlled by co-localization of three main PPCs leading to formation of thylakoid membrane mosaic. This organization might represent evolutional and functional precursor for the granal/stromal spatial heterogeneity in photosystems that is typical for higher plant thylakoids.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2020
    Elektronická adresahttps://www.sciencedirect.com/science/article/pii/S0005272819300908?via%3Dihub
Počet záznamů: 1  

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