Počet záznamů: 1
Transcription of specific auxin efflux and influx carriers drives auxin homeostasis in tobacco cells
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SYSNO ASEP 0517328 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Transcription of specific auxin efflux and influx carriers drives auxin homeostasis in tobacco cells Tvůrce(i) Müller, Karel (UEB-Q) RID, ORCID
Hošek, Petr (UEB-Q) ORCID
Laňková, Martina (UEB-Q) RID, ORCID
Vosolsobě, S. (CZ)
Malínská, Kateřina (UEB-Q) RID, ORCID
Čarná, Mária (UEB-Q)
Fílová, Markéta (UEB-Q)
Dobrev, Petre (UEB-Q) RID, ORCID
Helusová, Michaela (UEB-Q)
Hoyerová, Klára (UEB-Q) RID, ORCID
Petrášek, Jan (UEB-Q) RID, ORCIDCelkový počet autorů 11 Zdroj.dok. Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 100, č. 3 (2019), s. 627-640Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova aux1/lax ; auxin efflux carrier ; auxin homeostasis ; auxin influx carrier ; auxin transport ; Nicotiana tabacum ; pin ; tobacco BY-2 cells Vědní obor RIV ED - Fyziologie Obor OECD Biochemical research methods CEP EF16_013/0001775 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA16-10948S GA ČR - Grantová agentura ČR GA16-19557S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000484570800001 EID SCOPUS 85071447539 DOI 10.1111/tpj.14474 Anotace Auxin concentration gradients are informative for the transduction of many developmental cues, triggering downstream gene expression and other responses. The generation of auxin gradients depends significantly on cell-to-cell auxin transport, which is supported by the activities of auxin efflux and influx carriers. However, at the level of individual plant cell, the co-ordination of auxin efflux and influx largely remains uncharacterized. We addressed this issue by analyzing the contribution of canonical PIN-FORMED (PIN) proteins to the carrier-mediated auxin efflux in Nicotiana tabacum L., cv. Bright Yellow (BY-2) tobacco cells. We show here that a majority of canonical NtPINs are transcribed in cultured cells and in planta. Cloning of NtPIN genes and their inducible overexpression in tobacco cells uncovered high auxin efflux activity of NtPIN11, accompanied by auxin starvation symptoms. Auxin transport parameters after NtPIN11 overexpression were further assessed using radiolabelled auxin accumulation and mathematical modelling. Unexpectedly, these experiments showed notable stimulation of auxin influx, which was accompanied by enhanced transcript levels of genes for a specific auxin influx carrier and by decreased transcript levels of other genes for auxin efflux carriers. A similar transcriptional response was observed upon removal of auxin from the culture medium, which resulted in decreased auxin efflux. Overall, our results revealed an auxin transport-based homeostatic mechanism for the maintenance of endogenous auxin levels. Open Research Badges: This article has earned an Open Data Badge for making publicly available the digitally-shareable data necessary to reproduce the reported results. The data is available at http://osf.io/ka97b/. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2020 Elektronická adresa http://dx.doi.org/10.1111/tpj.14474
Počet záznamů: 1