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Ubiquitin-proteasome system participates in the de-aggregation of spermadhesins and DQH protein during boar sperm capacitation
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SYSNO ASEP 0510859 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ubiquitin-proteasome system participates in the de-aggregation of spermadhesins and DQH protein during boar sperm capacitation Tvůrce(i) Zigo, M. (US)
Jonáková, Věra (BTO-N) RID
Maňásková-Postlerová, Pavla (BTO-N) ORCID
Kerns, K. (US)
Sutovsky, P. (US)Celkový počet autorů 5 Zdroj.dok. Reproduction. - : BioScientifica - ISSN 1470-1626
Roč. 157, č. 3 (2019), s. 283-295Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova SEMINAL PLASMA-PROTEINS ; SURFACE-PROTEINS ; ZONA-PELLUCIDA Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Reproductive biology (medical aspects to be 3) CEP GA18-11275S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000457578600011 EID SCOPUS 85065722693 DOI 10.1530/REP-18-0413 Anotace We studied the participation of the ubiquitin-proteasome system (UPS) in spermadhesin release during in vitro capacitation (IVC) of domestic boar spermatozoa. At ejaculation, boar spermatozoa acquire low molecular weight (8-16 kDa) seminal plasma proteins, predominantly spermadhesins, aggregated on the sperm surface. Due to their arrangement, such aggregates are relatively inaccessible to antibody labeling. As a result of de-aggregation and release of the outer layers of spermadhesins from the sperm surface during IVC, antibody labeling becomes feasible in the capacitated spermatozoa. In vivo, the capacitation-induced shedding of spermadhesins from the sperm surface is associated with the release of spermatozoa from the oviductal sperm reservoir. We took advantage of this property to perform image-based flow cytometry to study de-aggregation and shedding of boar spermadhesins (AQN, AWN, PSP protein families) and boar DQH (BSP1) sperm surface protein which induces higher fluorescent intensity in capacitated vs ejaculated spermatozoa. Addition of a proteasomal inhibitor (100 pM MG132) during IVC significantly reduced fluorescence intensity of all studied proteins (P< 0.05) compared to vehicle control IVC. Western blot detection of spermadhesins did not support their retention during IVC with proteasomal inhibition (P> 0.99) but showed the accumulation of DQH (P=0.03) during IVC, compared to vehicle control IVC. Our results thus demonstrate that UPS participates in the de-aggregation of spermadhesins and DQH protein from the sperm surface during capacitation, with a possible involvement in sperm detachment from the oviductal sperm reservoir and/or sperm-zona pellucida interactions. The activity of sperm UPS modulates de-aggregation of boar spermadhesins and DQH sperm surface protein/binder of sperm1 (BSP1) during the sperm capacitation. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2020 Elektronická adresa https://rep.bioscientifica.com/view/journals/rep/157/3/REP-18-0413.xml
Počet záznamů: 1