Počet záznamů: 1
Chemically-induced DNA de-methylation alters the effectiveness of microspore embryogenesis in triticale
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SYSNO ASEP 0508704 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Chemically-induced DNA de-methylation alters the effectiveness of microspore embryogenesis in triticale Tvůrce(i) Nowicka, Anna (UEB-Q) ORCID
Juzoń, K. (PL)
Krzewska, M. (PL)
Dziurka, M. (PL)
Dubas, E. (PL)
Kopeć, P. (PL)
Zieliński, K. (PL)
Żur, I. (PL)Celkový počet autorů 8 Číslo článku 110189 Zdroj.dok. Plant Science. - : Elsevier - ISSN 0168-9452
Roč. 287, Oct (2019)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. IE - Irsko Klíč. slova 2′-deoxy-5-azacytidine ; 5-azacytidine ; Cytotoxicity ; DNA methylation ; Double haploid ; ×Triticosecale Wittm Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Plant sciences, botany CEP GA18-12197S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000487165500020 EID SCOPUS 85069590359 DOI https://doi.org/10.1016/j.plantsci.2019.110189 Anotace Microspores exposed to some stress factors may display cell totipotency and could be reprogrammed towards embryogenic development. Plant breeding and genetic engineering widely use haploids/doubled haploids (DHs) derived from in vitro-cultured microspores, but the mechanism of this process remains poorly understood. Recently published data suggest that microspore embryogenesis (ME) is accompanied by changes in DNA methylation and chromatin reorganization. Here, we used two triticale DH lines (DH19 and DH28), significantly different with respect to embryogenic potential. To change DNA methylation levels, we applied two cytosine-analogs: 5-azacytidine (AC) and 2′-deoxy-5-azacytidine (DAC) treatments. We found that chemically-induced DNA demethylation caused chromatin relaxation and dysregulation of marker genes (TaTPD1-like, GSTF2, GSTA2, CHI3, Tad1, TaNF-YA7, SERK2, TaME1) related to ME. Both drugs showed significant cytotoxicity in a dose-dependent manner. We noticed that lines varied in terms of overall DNA methylation levels and responded in a different way to hypomethylation caused by the drugs. DH19 (low embryogenic) after inhibitors treatment, showed higher microspore viability, but its recalcitrancy was not overcome. For highly embryogenic DH28, we noted significantly higher effectiveness of embryo-like structure production and plant regeneration. In summary, our study provides new insight into the role of DNA methylation in ME initiation. They suggest potential benefits resulting from the utilization of epigenetic inhibitors to improve the process of DHs production. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2020 Elektronická adresa http://dx.doi.org/10.1016/j.plantsci.2019.110189
Počet záznamů: 1