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Effect of iron oxide nanoparticles with ascorbic acid on neural stem cells
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SYSNO ASEP 0507370 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Effect of iron oxide nanoparticles with ascorbic acid on neural stem cells Tvůrce(i) Jiráková, Klára (UEM-P)
Moskvin, Maksym (UMCH-V) RID, ORCID
Horák, Daniel (UMCH-V) RID, ORCID
Jendelová, Pavla (UEM-P) RID, ORCIDZdroj.dok. Nanocon 2017 : conference proceedings : 9th International Conference on Nanomaterials - Research & Application. - Ostrava : Tanger Ltd., 2018 - ISBN 9788087294819 Rozsah stran s. 668-673 Poč.str. 6 s. Forma vydání Tištěná - P Akce International Conference on Nanomaterials - Research and Application (NANOCON) /9./ Datum konání 18.10.2017 - 20.10.2017 Místo konání Brno Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova neural stem cells ; nanoparticles ; oxidative stress ; ascorbic acid Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Neurosciences (including psychophysiology Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Makromolekulární chemie CEP LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 ; UMCH-V - RVO:61389013 UT WOS 000452823300110 EID SCOPUS 85051847953 Anotace Cells labelled with iron oxide nanoparticles (ION) can be tracked by magnetic resonance imaging (MRI) in several applications. However, various studies demonstrated toxicity and oxidative stress induction associated with nanoparticles exposure. We analysed biologic effects after the exposure of two types of iron oxide nanoparticles (with and without an antioxidative agent, an ascorbic acid) on human neural stem cells. The labelled cells in gel phantoms were detected in MRI and they showed decreased relaxation rates in comparison with control. ION slightly decreased cell proliferation in comparison with unlabelled cells, which was dependent on concentration and presence of ascorbic acid. None of the nanoparticle type showed negative effect on cell viability and both demonstrated minor effect on reactive oxygen species (ROS) formation. Unfortunately, ascorbic acid bound to nanoparticles did not show any effect on ROS attenuation. Cells exposed to both types of nanoparticles showed increased positivity for a phosphorylated form of H2AX a marker of double strand breaks. We showed that ION in low concentrations do not affect cell viability, but have negative effect on cells on DNA level. Their potential use for oxidative stress reduction is dependent on the concentration of ascorbic acid bound to the nanoparticles and this should be further increased. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2020
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