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CPEB2 Is Necessary for Proper Porcine Meiotic Maturation and Embryonic Development
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SYSNO ASEP 0506488 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název CPEB2 Is Necessary for Proper Porcine Meiotic Maturation and Embryonic Development Tvůrce(i) Procházková, Barbora (UZFG-Y)
Komrsková, Pavla (UZFG-Y) RID
Kubelka, Michal (UZFG-Y) RID, ORCIDČíslo článku 3138 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 19, č. 10 (2018)Poč.str. 19 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova oocyte maturation ; embryonic development ; translational control Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology CEP GA15-22765S GA ČR - Grantová agentura ČR EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000448951000293 EID SCOPUS 85054897490 DOI 10.3390/ijms19103138 Anotace Oocyte meiotic maturation and embryogenesis are some of the most important physiological processes that occur in organisms, playing crucial roles in the preservation of life in all species. The post-transcriptional regulation of maternal messenger ribonucleic acids (mRNAs) and the post-translational regulation of proteins are critical in the control of oocyte maturation and early embryogenesis. Translational control affects the basic mechanism of protein synthesis, thus, knowledge of the key components included in this machinery is required in order to understand its regulation. Cytoplasmic polyadenylation element binding proteins (CPEBs) bind to the 3-end of mRNAs to regulate their localization and translation and are necessary for proper development. In this study we examined the expression pattern of cytoplasmic polyadenylation element binding protein 2 (CPEB2) both on the mRNA (by real-time quantitative reverse transcription polymerase chain reaction, qRT-PCR) and protein (by Western blotting, WB) level, as well as its localization during the meiotic maturation of porcine oocytes and early embryonic development by immunocytochemistry (ICC). For the elucidation of its functions, CPEB2 knockdown by double-strand RNA (dsRNA) was used. We discovered that CPEB2 is expressed during all stages of porcine meiotic maturation and embryonic development. Moreover, we found that it is necessary to enable a high percentage of oocytes to reach the metaphase II (MII) stage, as well as for the production of good-quality parthenogenetic blastocysts. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://www.mdpi.com/1422-0067/19/10/3138
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