Počet záznamů: 1
Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine
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SYSNO ASEP 0506160 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine Tvůrce(i) Patsula, Vitalii (UMCH-V) RID, ORCID
Tulinská, J. (SK)
Trachtová, Š. (CZ)
Kuricová, M. (SK)
Lišková, A. (SK)
Španová, A. (CZ)
Čiampor, F. (SK)
Vavra, I. (SK)
Rittich, B. (CZ)
Ursinyova, M. (SK)
Dusinska, M. (NO)
Ilavská, S. (SK)
Horváthová, M. (SK)
Masanova, V. (SK)
Uhnakova, I. (SK)
Horák, Daniel (UMCH-V) RID, ORCIDZdroj.dok. Nanotoxicology. - : Taylor & Francis - ISSN 1743-5390
Roč. 13, č. 4 (2019), s. 510-526Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova iron oxide nanoparticles ; PCR inhibition ; cell interaction and uptake Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science CEP GA17-04918S GA ČR - Grantová agentura ČR LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000477976100006 EID SCOPUS 85060941650 DOI 10.1080/17435390.2018.1555624 Anotace Innovative nanotechnology aims to develop particles that are small, monodisperse, smart, and do not cause unintentional side effects. Uniform magnetic Fe3O4 nanoparticles (12 nm in size) were prepared by thermal decomposition of iron(III) oleate. To make them colloidally stable and dispersible in water and cell culture medium, they were modified with phosphonic acid- (PA) and hydroxamic acid (HA)-terminated poly(ethylene glycol) yielding PA-PEG@Fe3O4 and HA-PEG@Fe3O4 nanoparticles. Conventional gama-Fe2O3 particles were prepared as a control. Advanced techniques were used to evaluate the properties and safety of the particles. Completeness of the nanoparticle coating was tested by real-time polymerase chain reaction. Interaction of the particles with primary human peripheral blood cells, cellular uptake, cytotoxicity, and immunotoxicity were also investigated. Amount of internalized iron in peripheral blood mononuclear cells was 72, 38, and 25 pg Fe/cell for HA-PEG@Fe3O4,gama-Fe2O3, and PA-PEG@Fe3O4, respectively. Nanoparticles were localized within the cytoplasm and in the extracellular space. No cytotoxic effect of both PEGylated nanoparticles was observed (0.12–75 μg/cm2) after 24 and 72-h incubation. Moreover, no suppressive effect was found on the proliferative activity of T-lymphocytes and T-dependent B-cell response, phagocytic activity of monocytes and granulocytes, and respiratory burst of phagocytes. Similarly, no cytotoxic effect of gama-Fe2O3 particles was observed. However, they suppressed the proliferative activity of T-lymphocytes (75 μg/cm2, 72 h) and also decreased the phagocytic activity of monocytes (15 μg/cm2 24 h, 3–75 μg/cm2 72 h). We thus show that newly developed particles have great potential especially in cancer diagnostics and therapy. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2020 Elektronická adresa https://www.tandfonline.com/doi/full/10.1080/17435390.2018.1555624
Počet záznamů: 1