Počet záznamů: 1
Anti-arrhythmic Cardiac Phenotype Elicited by Chronic Intermittent Hypoxia Is Associated With Alterations in Connexin-43 Expression, Phosphorylation, and Distribution
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SYSNO ASEP 0504257 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Anti-arrhythmic Cardiac Phenotype Elicited by Chronic Intermittent Hypoxia Is Associated With Alterations in Connexin-43 Expression, Phosphorylation, and Distribution Tvůrce(i) Kohutová, J. (CZ)
Elsnicová, B. (CZ)
Holzerová, Kristýna (FGU-C) ORCID, RID
Neckář, Jan (FGU-C) RID, ORCID
Šebesta, O. (CZ)
Ježková, J. (CZ)
Vecka, M. (CZ)
Vebr, P. (CZ)
Horníková, D. (CZ)
Szeiffová Bačová, B. (SK)
Egan Beňová, T. (SK)
Hlaváčková, Markéta (FGU-C) RID, ORCID
Tribulová, N. (SK)
Kolář, František (FGU-C) RID, ORCID, SAI
Nováková, Olga (FGU-C)
Žurmanová, J.M. (CZ)Číslo článku 789 Zdroj.dok. Frontiers in Endocrinology. - : Frontiers Media - ISSN 1664-2392
Roč. 9, Jan 25 (2019)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova heart ; chronic hypoxia ; brief ischemia ; arrhythmia ; connexin-43 ; n-3 PUFA Vědní obor RIV ED - Fyziologie Obor OECD Physiology (including cytology) CEP GA17-07748S GA ČR - Grantová agentura ČR GJ16-12420Y GA ČR - Grantová agentura ČR LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000456727200001 EID SCOPUS 85064200437 DOI 10.3389/fendo.2018.00789 Anotace Remodeling of the cellular distribution of gap junctions formed mainly by connexin-43 (Cx43) can be related to the increased incidence of cardiac arrhythmias. It has been shown that adaptation to chronic intermittent hypobaric hypoxia (IHH) attenuates the incidence and severity of ischemic and reperfusion ventricular arrhythmias and increases the proportion of anti-arrhythmic n-3 polyunsaturated fatty acids (n-3 PUFA) in heart phospholipids. Wistar rats were exposed to simulated IHH (7,000 m, 8-h/day, 35 exposures) and compared with normoxic controls (N). Cx43 expression, phosphorylation, localization and n-3 PUFA proportion were analyzed in left ventricular myocardium. Compared to N, IHH led to higher expression of total Cx43, its variant phosphorylated at Ser368 [p-Cx43(Ser368)], which maintains “end to end” communication, as well as p-Cx43(Ser364/365), which facilitates conductivity. By contrast, expression of non-phosphorylated Cx43 and p-Cx43(Ser278/289), attenuating intercellular communication, was lower in IHH than in N. IHH also resulted in increased expression of protein kinase A and protein kinase G while casein kinase 1 did not change compared to N. In IHH group, which exhibited reduced incidence of ischemic ventricular arrhythmias, Cx43 and p-Cx43(Ser368) were more abundant at “end to end” gap junctions than in N group and this difference was preserved after acute regional ischemia (10 min). We further confirmed higher n-3 PUFA proportion in heart phospholipids after adaptation to IHH, which was even further increased by ischemia. Our results suggest that adaptation to IHH alters expression, phosphorylation and distribution of Cx43 as well as cardioprotective n-3PUFA proportion suggesting that the anti-arrhythmic phenotype elicited by IHH can be at least partly related to the stabilization of the “end to end” conductivity between cardiomyocytes during brief ischemia. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2020 Elektronická adresa https://doi.org/10.3389/fendo.2018.00789
Počet záznamů: 1