Počet záznamů: 1
Parallel in vitro and in silico investigations into anti-inflammatory effects of non-prenylated stilbenoids
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SYSNO ASEP 0502554 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Parallel in vitro and in silico investigations into anti-inflammatory effects of non-prenylated stilbenoids Tvůrce(i) Leláková, V. (CZ)
Šmejkal, K. (CZ)
Jakubczyk, Karolína (UEB-Q)
Veselý, Ondřej (UEB-Q)
Landa, Přemysl (UEB-Q) RID, ORCID
Václavík, J. (CZ)
Bobáľ, P. (CZ)
Pížová, H. (CZ)
Temml, V. (AT)
Steinacher, T. (AT)
Schuster, D. (AT)
Granica, S. (PL)
Hanáková, Z. (CZ)
Hošek, J. (CZ)Celkový počet autorů 14 Zdroj.dok. Food Chemistry. - : Elsevier - ISSN 0308-8146
Roč. 285, JUL 1 (2019), s. 431-440Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Anti-inflammatory ; Cyclooxygenase ; Lipoxygenase ; Macrophages ; Molecular docking simulations ; NF-κB ; Stilbenes Vědní obor RIV GM - Potravinářství Obor OECD Biochemical research methods CEP GA16-07193S GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000459823800053 EID SCOPUS 85061429064 DOI 10.1016/j.foodchem.2019.01.128 Anotace Stilbenoids represent a large group of bioactive compounds, which occur in food and medicinal plants. Twenty-five stilbenoids were screened in vitro for their ability to inhibit COX-1, COX-2 and 5-LOX. Piceatannol and pinostilbene showed activity comparable to the zileuton and ibuprofen, respectively. The anti-inflammatory potential of stilbenoids was further evaluated using THP-1 human monocytic leukemia cell line. Tests of the cytotoxicity on the THP-1 and HCT116 cell lines showed very low toxic effects. The tested stilbenoids were evaluated for their ability to attenuate the LPS-stimulated activation of NF-κB/AP-1. Most of the tested substances reduced the activity of NF-κB/AP-1 and later attenuated the expression of TNF-α. The effects of selected stilbenoids were further investigated on inflammatory signaling pathways. Non-prenylated stilbenoids regulated attenuation of NF-ĸB/AP-1 activity upstream by inhibiting the phosphorylation of MAPKs. A docking study used to in silico analyze the tested compounds confirmed their interaction with NF-ĸB, COX-2 and 5-LOX. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2019
Počet záznamů: 1