Počet záznamů: 1
Asymmetric pitx2 expression in medaka epithalamus is regulated by nodal signaling through an intronic enhancer
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SYSNO ASEP 0502300 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Asymmetric pitx2 expression in medaka epithalamus is regulated by nodal signaling through an intronic enhancer Tvůrce(i) Soukup, Vladimír (UMG-J)
Mršťáková, Simona (UMG-J)
Kozmik, Zbyněk (UMG-J) RIDCelkový počet autorů 3 Zdroj.dok. Development Genes and Evolution - ISSN 0949-944X
Roč. 228, č. 2 (2018), s. 131-139Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Epithalamus ; Asymmetry ; Transgene ; Enhancer ; Medaka ; pitx2 Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology CEP GP14-20839P GA ČR - Grantová agentura ČR LO1419 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMG-J - RVO:68378050 UT WOS 000430538400005 DOI 10.1007/s00427-018-0611-1 Anotace The epithalamic region of fishes shows prominent left-right asymmetries that are executed by nodal signaling upstream of the asymmetry-determining transcription factor pitx2. Previous reports have identified that nodal controls the left-sided pitx2 expression in the lateral plate mesoderm through an enhancer present in the last intron of this gene. However, whether similar regulation occurs also in the case of epithalamic asymmetry is currently unresolved. Here, we address some of the cis-regulatory information that control asymmetric pitx2 expression in epithalamus by presenting a Tg(pitx2:EGFP) (116-17) transgenic medaka model, which expresses enhanced green fluorescent protein (EGFP) under control of an intronic enhancer. We show that this transgene recapitulates epithalamic expression of the endogenous pitx2 and that it responds to nodal signaling inhibition. Further, we identify that three foxh1-binding sites present in this enhancer modulate expression of the transgene and that the second site is absolutely necessary for the left-sided epithalamic expression while the other two sites may have subtler regulative roles. We provide evidence that left-sided epithalamic pitx2 expression is controlled through an enhancer present in the last intron of this gene and that the regulatory logic underlying asymmetric pitx2 expression is shared between epithalamic and lateral plate mesoderm regions. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2019
Počet záznamů: 1