Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

Grybchuk, D.; Akopyants, N.S.; Kostygov, A. Y.; Konovalovas, A.; Lye, L.-F.; Dobson, D.E.; Zangger, H.; Fasel, N.; Butenko, A.; Frolov, A. O.; Votýpka, Jan; d'Avila-Levy, C.M.; Kulich, P.; Moravcová, J.; Plevka, P.; Rogozin, I.B.; Serva, S.; Lukeš, Julius; Beverley, S.M.; Yurchenko, Vyacheslav

doi:https://dx.doi.org/10.1073/pnas.1717806115

Počet záznamů: 1

Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

1.

SYSNO ASEP	0498650
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania
Tvůrce(i)	Grybchuk, D. (CZ) Akopyants, N.S. (US) Kostygov, A. Y. (RU) Konovalovas, A. (LT) Lye, L.-F. (US) Dobson, D.E. (US) Zangger, H. (CH) Fasel, N. (CH) Butenko, A. (CZ) Frolov, A. O. (RU) Votýpka, Jan (BC-A)_{RID, ORCID} d'Avila-Levy, C.M. (BR) Kulich, P. (CZ) Moravcová, J. (CZ) Plevka, P. (CZ) Rogozin, I.B. (US) Serva, S. (LT) Lukeš, Julius (BC-A)_{RID, ORCID} Beverley, S.M. (US) Yurchenko, Vyacheslav (BC-A)_{RID, ORCID}
Celkový počet autorů	20
Zdroj.dok.	Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424 Roč. 115, č. 3 (2018), E506-E515
Poč.str.	10 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	Trypanosomatidae ; Bunyavirales ; persistent virus infection ; coinfection ; coevolution
Vědní obor RIV	EB - Genetika a molekulární biologie
Obor OECD	Genetics and heredity (medical genetics to be 3)
CEP	GA16-18699S GA ČR - Grantová agentura ČR
Institucionální podpora	BC-A - RVO:60077344
UT WOS	000423091400021
EID SCOPUS	85042099563
DOI	10.1073/pnas.1717806115
Anotace	Knowledge of viral diversity is expanding greatly, but many lineages remain underexplored. We surveyed RNA viruses in 52 cultured monoxenous relatives of the human parasite Leishmania (Crithidia and Leptomonas), as well as plant-infecting Phytomonas. Leptomonas pyrrhocoris was a hotbed for viral discovery, carrying a virus (Leptomonas pyrrhocoris ostravirus 1) with a highly divergent RNA-dependent RNA polymerase missed by conventional BLAST searches, an emergent clade of tombus-like viruses, and an example of viral endogenization. A deep-branching clade of trypanosomatid narnaviruses was found, notable as Leptomonas seymouri bearing Narna-like virus 1 (LepseyNLV1) have been reported in cultures recovered from patients with visceral leishmaniasis. A deep-branching trypanosomatid viral lineage showing strong affinities to bunyaviruses was termed ´Leishbunyavirus´ (LBV) and judged sufficiently distinct to warrant assignment within a proposed family termed ´Leishbunyaviridae.´ Numerous relatives of trypanosomatid viruses were found in insect metatranscriptomic surveys, which likely arise from trypanosomatid microbiota. Despite extensive sampling we found no relatives of the totivirus Leishmaniavirus (LRV1/2), implying that it was acquired at about the same time the Leishmania became able to parasitize vertebrates. As viruses were found in over a quarter of isolates tested, many more are likely to be found in the >600 unsurveyed trypanosomatid species. Viral loss was occasionally observed in culture, providing potentially isogenic virus-free lines enabling studies probing the biological role of trypanosomatid viruses. These data shed important insights on the emergence of viruses within an important trypanosomatid clade relevant to human disease.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2019

Počet záznamů: 1