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Hidden in plain sight-highly abundant and diverse planktonic freshwater Chloroflexi
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SYSNO ASEP 0495144 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Hidden in plain sight-highly abundant and diverse planktonic freshwater Chloroflexi Tvůrce(i) Mehrshad, Maliheh (BC-A) RID
Salcher, M.M. (CH)
Okazaki, Y. (JP)
Nakano, S. (JP)
Šimek, Karel (BC-A) RID, ORCID
Andrei, Adrian-Stefan (BC-A) RID, ORCID
Ghai, Rohit (BC-A) RID, ORCIDČíslo článku 176 Zdroj.dok. Microbiome. - : BioMed Central - ISSN 2049-2618
Roč. 6, OCT (2018)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Chloroflexi ; freshwater ecology ; metagenomics ; CARD-FISH Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA17-04828S GA ČR - Grantová agentura ČR GA13-00243S GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000446308400001 EID SCOPUS 85054339917 DOI 10.1186/s40168-018-0563-8 Anotace Background: Representatives of the phylum Chloroflexi, though reportedly highly abundant in the extensive deep water habitats of both marine (SAR202 up to 30% of total prokaryotes) and freshwater (CL500-11 up to 26% of total prokaryotes), remain uncultivated and uncharacterized. There are few metagenomic studies on marine Chloroflexi representatives, while the pelagic freshwater Chloroflexi community is largely unknown except for a single metagenome-assembled genome of CL500-11. Results: Here, we provide the first extensive examination of the community composition of this cosmopolitan phylum in a range of pelagic habitats (176 datasets) and highlight the impact of salinity and depth on their phylogenomic composition. Reconstructed genomes (53 in total) provide a perspective on the phylogeny, metabolism, and distribution of three novel classes and two family-level taxa within the phylum Chloroflexi. We unraveled a remarkable genomic diversity of pelagic freshwater Chloroflexi representatives that thrive not only in the hypolimnion as previously suspected, but also in the epilimnion. Our results suggest that the lake hypolimnion provides a globally stable habitat reflected in lower species diversity among hypolimnion-specific CL500-11 and TK10 clusters in distantly related lakes compared to a higher species diversity of the epilimnion-specific SL56 cluster. Cell volume analyses show that the CL500-11 are among the largest prokaryotic cells in the water column of deep lakes and with a biomass to abundance ratio of two they significantly contribute to the deep lake carbon flow. Metabolic insights indicate participation of JG30-KF-CM66 representatives in the global cobalamin production via cobinamide to cobalamin salvage pathway. Conclusions: Extending phylogenomic comparisons to brackish and marine habitats suggests salinity as the major influencer of the community composition of the deep-dwelling Chloroflexi in marine (SAR202) and freshwater (CL500-11) habitats as both counterparts thrive in intermediate brackish salinity, however, freshwater habitats harbor the most phylogenetically diverse community of pelagic Chloroflexi representatives that reside both in epi- and hypolimnion. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2019
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