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Encyclopedia of Biophysics
- 1.
SYSNO ASEP 0494619 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Fluorescence Correlation Spectroscopy of Lipid Membranes Tvůrce(i) Macháň, Radek (UFCH-W)
Hof, Martin (UFCH-W) RID, ORCIDZdroj.dok. Encyclopedia of Biophysics. - Geneve : Springer Nature Switzerland AG, 2018 - ISBN 978-3-642-35943-9 Rozsah stran s. 1-8 Poč.str. 34 s. Poč.str.knihy 1000 Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova fluorescence ; spectroscopy ; lipid membranes Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Obor OECD Physical chemistry Institucionální podpora UFCH-W - RVO:61388955 DOI https://doi.org/10.1007/978-3-642-35943-9_560-1 Anotace Fluorescence correlation spectroscopy (FCS), along with fluorescence recovery after photobleaching (FRAP) and single particle tracking (SPT), belongs among the most important techniques for investigating molecular diffusion in biological systems, including lipid membranes. FCS extracts information on the kinetics of molecular movements and chemical reactions from the analysis of fluctuations in fluorescence intensity collected from a small observation volume. When applied to study mobility of molecules in biological membranes and their artificial models, it has to be adapted to the slow mobility of membrane components and the locally planar geometry. In biological membranes, their highly inhomogeneous structure ought to be considered as well. According to current models, cell membranes contain nanoscopic domains differing in composition and mechanical properties from their surroundings. Furthermore, interaction with cytoskeleton forms semipermeable barriers to membrane... Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2019 Elektronická adresa https://link.springer.com/referenceworkentry/10.1007/978-3-642-35943-9_560-1
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