Počet záznamů: 1
Integration of Genetic and Cytogenetic Maps and Identification of Sex Chromosome in Garden Asparagus (Asparagus officinalis L.)
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SYSNO ASEP 0493453 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Integration of Genetic and Cytogenetic Maps and Identification of Sex Chromosome in Garden Asparagus (Asparagus officinalis L.) Tvůrce(i) Moreno, R. (ES)
Castro, P. (ES)
Vrána, Jan (UEB-Q) RID, ORCID
Kubaláková, Marie (UEB-Q) RID
Cápal, Petr (UEB-Q) RID, ORCID
Garcia, V. (ES)
Gil, J. (ES)
Millán, T. (ES)
Doležel, Jaroslav (UEB-Q) RID, ORCIDCelkový počet autorů 9 Číslo článku 1068 Zdroj.dok. Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 9, JUL 31 (2018)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova flow-sorted chromosomes ; microsatellite markers ; plant chromosome ; physical maps ; linkage map ; vicia-faba ; genome ; barley ; locus ; cytometry ; Asparagus officinalis ; sex chromosome ; fish ; flow-sorted chromosomes ; SNPs ; SSRs ; genetic map Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GBP501/12/G090 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000440277000001 EID SCOPUS 85051867327 DOI 10.3389/fpls.2018.01068 Anotace A genetic linkage map of dioecious garden asparagus (Asparagus officinalis L., 2n = 2x = 20) was constructed using F-1 population, simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. In total, 1376 SNPs and 27 SSRs were used for genetic mapping. Two resulting parental maps contained 907 and 678 markers spanning 1947 and 1814 cM, for female and male parent, respectively, over ten linkage groups representing ten haploid chromosomes of the species. With the aim to anchor the ten genetic linkage groups to individual chromosomes and develop a tool to facilitate genome analysis and gene cloning, we have optimized a protocol for flow cytometric chromosome analysis and sorting in asparagus. The analysis of DAPI-stained suspensions of intact mitotic chromosomes by flow cytometry resulted in histograms of relative fluorescence intensity (flow karyotypes) comprising eight major peaks. The analysis of chromosome morphology and localization of 5S and 45S rDNA by FISH on flow-sorted chromosomes, revealed that four chromosomes (IV, V, VI, VIII) could be discriminated and sorted. Seventy-two SSR markers were used to characterize chromosome content of individual peaks on the flow karyotype. Out of them, 27 were included in the genetic linkage map and anchored genetic linkage groups to chromosomes. The sex determining locus was located on LG5, which was associated with peak V representing a chromosome with 5S rDNA locus. The results obtained in this study will support asparagus improvement by facilitating targeted marker development and gene isolation using flow-sorted chromosomes. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2019
Počet záznamů: 1