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Does BCA3 Play a Role in the HIV-1 Replication Cycle?
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SYSNO ASEP 0491247 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Does BCA3 Play a Role in the HIV-1 Replication Cycle? Tvůrce(i) Rumlová, M. (CZ)
Křížová, I. (CZ)
Zelenka, J. (CZ)
Weber, Jan (UOCHB-X) RID, ORCID
Ruml, T. (CZ)Číslo článku 212 Zdroj.dok. Viruses. - : MDPI
Roč. 10, č. 4 (2018)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova HIV-1 ; BCA3 ; AKIP-1 ; M-PMV ; virus incorporation ; PKAc Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Virology Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000435184400073 EID SCOPUS 85046093716 DOI 10.3390/v10040212 Anotace The cellular role of breast carcinoma-associated protein (BCA3), also known as A-kinase-interacting protein 1 (AKIP-1), is not fully understood. Recently, we reported that full-length, but not C-terminally truncated, BCA3 is incorporated into virions of Mason-Pfizer monkey virus, and that BCA3 enhances HIV-1 protease-induced apoptosis. In the present study, we report that BCA3 is associated with purified and subtilisin-treated HIV particles. Using a combination of immune-based methods and confocal microscopy, we show that the C-terminus of BCA3 is required for packaging into HIV-1 particles. However, we were unable to identify an HIV-1 binding domain for BCA3, and we did not observe any effect of incorporated BCA3 on HIV-1 infectivity. Interestingly, the BCA3 C-terminus was previously identified as a binding site for the catalytic subunit of protein kinase A (PKAc), a cellular protein that is specifically packaged into HIV-1 particles. Based on our analysis of PKAc-BCA3 interactions, we suggest that BCA3 incorporation into HIV-1 particles is mediated by its ability to interact with PKAc. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2019 Elektronická adresa http://www.mdpi.com/1999-4915/10/4/212
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