Počet záznamů: 1
A Route to Superior Performance of a Nanoplasmonic Biosensor: Consideration of Both Photonic and Mass Transport Aspects
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SYSNO ASEP 0490475 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A Route to Superior Performance of a Nanoplasmonic Biosensor: Consideration of Both Photonic and Mass Transport Aspects Tvůrce(i) Špačková, Barbora (URE-Y)
Lynn, Nicholas Scott (URE-Y) RID
Slabý, Jiří (URE-Y)
Šípová, Hana (URE-Y)
Homola, Jiří (URE-Y) RIDCelkový počet autorů 5 Zdroj.dok. ACS Photonics. - : American Chemical Society - ISSN 2330-4022
Roč. 5, č. 3 (2018), s. 1019-1025Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova DNA detection ; Affinity biosensing ; nanoplasmonics Vědní obor RIV BO - Biofyzika Obor OECD Biophysics CEP GA15-06785S GA ČR - Grantová agentura ČR Institucionální podpora URE-Y - RVO:67985882 UT WOS 000428356400047 EID SCOPUS 85044317759 DOI https://doi.org/10.1021/acsphotonics.7b01319 Anotace Optical biosensors based on plasmonic nano structures present a promising alternative to conventional biosensing methods and provide unmatched possibilities for miniaturization and high-throughput analysis. Previous works on the topic, however, have been overwhelmingly directed toward elucidating the optical performance of such sensors, with little emphasis on the topic of mass transport. To date, there exists no examination, experimental nor theoretical, of the bioanalytical performance of such sensors (in terms of detection limits) that simultaneously addresses both optical and mass transport aspects in a quantitative manner. In this work we present a universal model that describes the smallest concentration that can be detected by a nanoplasmonic biosensor. Accounting for both optical and mass transport aspects, this model establishes a relationship between bioanalytical performance and the biosensor's design parameters. We employ the model to optimize the performance of a nanoplasmonic DNA biosensor consisting of randomly distributed gold nanorods on a glass substrate. Through both experimental and theoretical results, we show that the proper design of a nanostructured sensing substrate is one that maximizes mass transport efficiency while preserving the quality of the optical readout. All results are compared with those obtained using a conventional SPR biosensor. We show that an optimized nanoplasmonic substrate allows for the detection of DNA at concentrations of an order of magnitude lower with respect to an SPR biosensor Pracoviště Ústav fotoniky a elektroniky Kontakt Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Rok sběru 2019
Počet záznamů: 1