Počet záznamů: 1
Establishment of a tagged variant of Lgr4 receptor suitable for functional and expression studies in the mouse
- 1.
SYSNO ASEP 0486598 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Establishment of a tagged variant of Lgr4 receptor suitable for functional and expression studies in the mouse Author(s) Kříž, Vítězslav (UMG-J)
Krausová, Michaela (UMG-J) RID
Burešová, Petra (UMG-J)
Dobeš, Jan (UMG-J)
Hrčkulák, Dušan (UMG-J)
Babošová, Oľga (UMG-J)
Švec, Jiří (UMG-J) RID
Kořínek, Vladimír (UMG-J) RIDNumber of authors 8 Source Title Transgenic Research. - : Springer - ISSN 0962-8819
Roč. 26, č. 5 (2017), s. 689-701Number of pages 13 s. Language eng - English Country NL - Netherlands Keywords Genome editing ; Hemagglutinin tag ; Knock-in ; R-spondin ; TALENs ; Wnt signaling Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects GA14-33952S GA ČR - Czech Science Foundation (CSF) LO1419 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015040 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0395 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) UT WOS 000410925700010 DOI https://doi.org/10.1007/s11248-017-0027-0 Annotation Leucine-rich repeat-containing G-protein-coupled receptor 4 (LGR4) is produced in a broad spectrum of mouse embryonic and adult tissues and its deficiency results in embryonal or perinatal lethality. The LGR4 function was mainly related to its potentiation of canonical Wnt signaling, however, several recent studies associate LGR4 with additional signaling pathways. To obtain a suitable tool for studying the signaling properties of Lgr4, we generated a tagged variant of the Lgr4 receptor using gene targeting in the mouse oocyte. The modified Lgr4 allele expresses the Lgr4 protein fused with a triple hemagglutinin (3HA) tag located at the extracellular part of the protein. The allele is fully functional, enabling tracking of Lgr4 expression in the mouse tissues. We also show that via surface labeling, the 3HA tag allows direct isolation and analysis of living Lgr4-positive cells obtained from the small intestinal crypts. Finally, the HA tag-specific antibody can be employed to characterize the biochemical features of Lgr4 and to identify possible biding partners of the protein in cells derived from various mouse tissues. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2018
Počet záznamů: 1