Počet záznamů: 1
Polycomb repression complex 2 is required for the maintenance of retinal progenitor cells and balanced retinal differentiation
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SYSNO ASEP 0486285 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Polycomb repression complex 2 is required for the maintenance of retinal progenitor cells and balanced retinal differentiation Tvůrce(i) Fujimura, Naoko (UMG-J)
Kuželová, Andrea (UMG-J)
Ebert, A. (AT)
Strnad, Hynek (UMG-J) RID
Láchová, Jitka (UMG-J)
Machoň, Ondřej (UMG-J) RID
Busslinger, M. (AT)
Kozmik, Zbyněk (UMG-J) RIDCelkový počet autorů 8 Zdroj.dok. Developmental Biology. - : Elsevier - ISSN 0012-1606
Roč. 433, č. 1 (2018), s. 47-60Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Retina ; Differentiation ; Polycomb ; Eed Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology CEP GA15-23675S GA ČR - Grantová agentura ČR LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015040 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED2.1.00/19.0395 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMG-J - RVO:68378050 UT WOS 000418394200005 DOI 10.1016/j.ydbio.2017.11.004 Anotace Polycomb repressive complexes maintain transcriptional repression of genes encoding crucial developmental regulators through chromatin modification. Here we investigated the role of Polycomb repressive complex 2 (PRC2) in retinal development by inactivating its key components Eed and Ezh2. Conditional deletion of Ezh2 resulted in a partial loss of PRC2 function and accelerated differentiation of Muller glial cells. In contrast, inactivation of Eed led to the ablation of PRC2 function at early postnatal stage. Cell proliferation was reduced and retinal progenitor cells were significantly decreased in this mutant, which subsequently caused depletion of Muller glia, bipolar, and rod photoreceptor cells, primarily generated from postnatal retinal progenitor cells. Interestingly, the proportion of amacrine cells was dramatically increased at postnatal stages in the Eed-deficient retina. In accordance, multiple transcription factors controlling amacrine cell differentiation were upregulated. Furthermore, ChIP-seq analysis showed that these deregulated genes contained bivalent chromatin (H3K27me3(+) H3K4me3(+)). Our results suggest that PRC2 is required for proliferation in order to maintain the retinal progenitor cells at postnatal stages and for retinal differentiation by controlling amacrine cell generation. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2018
Počet záznamů: 1