Počet záznamů: 1
The structure formed by inverted repeats in p53 response elements determines the transactivation activity of p53 protein
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SYSNO ASEP 0485751 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The structure formed by inverted repeats in p53 response elements determines the transactivation activity of p53 protein Tvůrce(i) Brázda, Václav (BFU-R) RID, ORCID
Čechová, Jana (BFU-R)
Battistin, M. (IT)
Coufal, Jan (BFU-R) ORCID
Jagelská, Eva (BFU-R)
Raimondi, I. (IT)
Inga, A. (IT)Celkový počet autorů 7 Zdroj.dok. Biochemical and Biophysical Research Communications. - : Elsevier - ISSN 0006-291X
Roč. 483, č. 1 (2017), s. 516-521Poč.str. 6 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova tumor-suppressor p53 ; cruciform structures ; dna-conformation Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA15-21855S GA ČR - Grantová agentura ČR Institucionální podpora BFU-R - RVO:68081707 UT WOS 000397259000080 DOI https://doi.org/10.1016/j.bbrc.2016.12.113 Anotace The TP53 gene is the most frequently mutated gene in human cancer and p53 protein plays a crucial role in gene expression and cancer protection. Its role is manifested by interactions with other proteins and DNA. p53 is a transcription factor that binds to DNA response elements (REs). Due to the palindromic nature of the consensus binding site, several p53-REs have the potential to form cruciform structures. However, the influence of cruciform formation on the activity of p53-REs has not been evaluated. Therefore, we prepared sets of p53-REs with identical theoretical binding affinity in their linear state, but different probabilities to form extra helical structures, for in vitro and in vivo analyses. Then we evaluated the presence of cruciform structures when inserted into plasmid DNA and employed a yeast-based assay to measure transactivation potential of these p53-REs cloned at a chromosomal locus in isogenic strains. We show that transactivation in vivo correlated more with relative propensity of an RE to form cruciforms than to its predicted in vitro DNA binding affinity for wild type p53. Structural features of p53-REs could therefore be an important determinant of p53 transactivation function. (C) 2016 Elsevier Inc. All rights reserved. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2018
Počet záznamů: 1