Počet záznamů: 1
Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism
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SYSNO ASEP 0484849 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism Tvůrce(i) Burgos, R.C.R. (NL)
Schoeman, J.C. (NL)
van Winden, L.J. (NL)
Červinková, Kateřina (URE-Y)
Ramautar, R. (NL)
van Wijk, E.P.A. (NL)
Cifra, Michal (URE-Y) RID, ORCID, SAI
Berger, R. (NL)
Hankemeier, T. (NL)
van der Greef, J. (NL)Celkový počet autorů 10 Číslo článku 1229 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 7, č. 1 (2017)Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova LEUKEMIA-CELL LINE ; PROTEIN-KINASE-C ; LIPID-PEROXIDATION Vědní obor RIV BH - Optika, masery a lasery Obor OECD Optics (including laser optics and quantum optics) CEP GA13-29294S GA ČR - Grantová agentura ČR Institucionální podpora URE-Y - RVO:67985882 UT WOS 000400189700006 EID SCOPUS 85018387430 DOI https://doi.org/10.1038/s41598-017-01229-x Anotace In recent years, excessive oxidative metabolism has been reported as a critical determinant of pathogenicity in many diseases. The advent of a simple tool that can provide a physiological readout of oxidative stress would be a major step towards monitoring this dynamic process in biological systems, while also improving our understanding of this process. Ultra-weak photon emission (UPE) has been proposed as a potential tool for measuring oxidative processes due to the association between UPE and reactive oxygen species. Here, we used HL-60 cells as an in vitro model to test the potential of using UPE as readout for dynamically monitoring oxidative stress after inducing respiratory burst. In addition, to probe for possible changes in oxidative metabolism, we performed targeted metabolomics on cell extracts and culture medium. Lastly, we tested the effects of treating cells with the NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI). Our results show that UPE can be used as readout for measuring oxidative stress metabolism and related processes Pracoviště Ústav fotoniky a elektroniky Kontakt Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Rok sběru 2018
Počet záznamů: 1