Počet záznamů: 1
Mutant Wars2 gene in spontaneously hypertensive rats impairs brown adipose tissue function and predisposes to visceral obesity
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SYSNO ASEP 0484413 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Mutant Wars2 gene in spontaneously hypertensive rats impairs brown adipose tissue function and predisposes to visceral obesity Tvůrce(i) Pravenec, Michal (FGU-C) RID, ORCID
Zídek, Václav (FGU-C) RID
Landa, Vladimír (FGU-C) RID
Mlejnek, Petr (FGU-C) RID, ORCID
Šilhavý, Jan (FGU-C) RID, ORCID
Šimáková, Miroslava (FGU-C) RID, ORCID
Trnovská, J. (CZ)
Škop, V. (CZ)
Marková, I. (CZ)
Malínská, H. (CZ)
Hüttl, M. (CZ)
Kazdová, L. (CZ)
Bardová, Kristina (FGU-C) RID, ORCID, SAI
Tauchmannová, Kateřina (FGU-C) RID, ORCID
Vrbacký, Marek (FGU-C) RID, ORCID
Nůsková, Hana (FGU-C) RID, ORCID
Mráček, Tomáš (FGU-C) RID, ORCID
Kopecký, Jan (FGU-C) RID, ORCID
Houštěk, Josef (FGU-C) RID, ORCIDZdroj.dok. Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 66, č. 6 (2017), s. 917-924Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova brown adipose tissue ; spontaneously hypertensive rat ; quantitative trait loci ; transgenic ; Wars2 gene ; mitochondrial proteosynthesis Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Endocrinology and metabolism (including diabetes, hormones) CEP GA13-04420S GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000419545200002 EID SCOPUS 85039166789 DOI https://doi.org/10.33549/physiolres.933811 Anotace Brown adipose tissue (BAT) plays an important role in lipid and glucose metabolism in rodents and possibly also in humans. Identification of genes responsible for BAT function would shed light on underlying pathophysiological mechanisms of metabolic disturbances. Recent linkage analysis in the BXH/HXB recombinant inbred (RI) strains, derived from Brown Norway (BN) and spontaneously hypertensive rats (SHR), identified two closely linked quantitative trait loci (QTL) associated with glucose oxidation and glucose incorporation into BAT lipids in the vicinity of Wars2 (tryptophanyl tRNA synthetase 2 (mitochondrial)) gene on chromosome 2. The SHR harbors L53F WARS2 protein variant that was associated with reduced angiogenesis and Wars2 thus represents a prominent positional candidate gene. In the current study, we validated this candidate as a quantitative trait gene (QTG) using transgenic rescue experiment. SHR-Wars2 transgenic rats with wild type Wars2 gene when compared to SHR, showed more efficient mitochondrial proteosynthesis and increased mitochondrial respiration, which was associated with increased glucose oxidation and incorporation into BAT lipids, and with reduced weight of visceral fat. Correlation analyses in RI strains showed that increased activity of BAT was associated with amelioration of insulin resistance in muscle and white adipose tissue. In summary, these results demonstrate important role of Wars2 gene in regulating BAT function and consequently lipid and glucose metabolism. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2018
Počet záznamů: 1