Počet záznamů: 1
The Stoichiometry of Isoquercitrin Complex with Iron or Copper Is Highly Dependent on Experimental Conditions
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SYSNO ASEP 0483712 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Stoichiometry of Isoquercitrin Complex with Iron or Copper Is Highly Dependent on Experimental Conditions Tvůrce(i) Catapano, M.C. (CZ)
Tvrdý, V. (CZ)
Karlíčková, J. (CZ)
Migkos, T. (CZ)
Valentová, Kateřina (MBU-M) RID, ORCID
Křen, Vladimír (MBU-M) RID, ORCID
Mladěnka, P. (CZ)Zdroj.dok. Nutrients. - : MDPI - ISSN 2072-6643
Roč. 9, č. 11 (2017), s. 1193Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova chelator ; copper ; quercetin-3-O-beta-glucopyranoside Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP LD15082 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000416547200031 EID SCOPUS 85032729211 DOI https://doi.org/10.3390/nu9111193 Anotace Interaction of flavonoids with transition metals can be partially responsible for their impact on humans. Stoichiometry of the iron/copper complex with a flavonoid glycoside isoquercitrin, a frequent component of food supplements, was assessed using competitive and non-competitive methods in four (patho)physiologically-relevant pH values (4.5. 5.5, 6.8, and 7.5). Isoquercitrin chelated all tested ions (Fe2+, Fe3+, Cu2+, and Cu+) but its affinity for Cu+ ions proved to be very low. In general, the chelation potency dropped with pH lowering. Metal complexes of 1:1 stoichiometry were mostly formed, however, they were not stable and the stoichiometry changed depending on conditions. Isoquercitrin was able to reduce both Cu2+ and Fe3+ ions at low ratios, but its reducing potential was diminished at higher ratios (isoquercitrin to metal) due to the metal chelation. In conclusion, this study emphasizes the need of using multiple different methods for the assessment of chelation potential in moderately-active metal chelators, like flavonoids. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
Počet záznamů: 1