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Carbon nanospecies affecting amyloid formation
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SYSNO ASEP 0481916 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Carbon nanospecies affecting amyloid formation Tvůrce(i) Holubová, Monika (UMCH-V) ORCID, RID
Konefal, Rafal (UMCH-V) RID, ORCID
Morávková, Zuzana (UMCH-V) RID, ORCID
Zhigunov, Alexander (UMCH-V) RID, ORCID
Svoboda, Jan (UMCH-V) ORCID, RID
Pop-Georgievski, Ognen (UMCH-V) RID, ORCID
Hromádková, Jiřina (UMCH-V) RID
Groborz, Ondřej (UMCH-V) ORCID, RID
Štěpánek, Petr (UMCH-V) RID, ORCID
Hrubý, Martin (UMCH-V) RID, ORCIDZdroj.dok. RSC Advances. - : Royal Society of Chemistry - ISSN 2046-2069
Roč. 7, č. 85 (2017), s. 53887-53898Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova amyloid fibril ; nanodiamond ; fullerene Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science CEP LM2015064 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NV16-30544A GA MZd - Ministerstvo zdravotnictví GA16-03156S GA ČR - Grantová agentura ČR TE01020118 GA TA ČR - Technologická agentura ČR LO1507 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000416831000028 EID SCOPUS 85035363583 DOI https://doi.org/10.1039/C7RA11296C Anotace Carbon nanospecies (CNPs) are of high interest in current research due to their many unique properties. They may be created by common processes, such as burning. Therefore, they can become potential contaminants and may have a negative impact on human health and the biosphere. Moreover, they may also catalyze protein misfolding and subsequent amyloid formation, which is extremely hard to treat. We investigated the influence of single-walled carbon nanotubes (SWNTs), fullerene (C60), carbon quantum dots (CDs) and nanodiamonds (NDs) on amyloid formation. This research utilized the hen egg-white lysozyme (HEWL) as a model system. Fibrils were detected by fluorescence of thioflavin-T (ThT) or Nile red (NR) and the results were confirmed by transmission electron microscopy (TEM). We have found that NDs promoted amyloid fibril formation at all concentrations. The highest concentration of C60 (250 µg ml-1) accelerated the process of fibrillation, while smaller concentrations (16 and 80 µg ml-1) prolonged the lag phase and were comparable to the control. SWNTs prolonged the lag phase of amyloid formation at all concentrations. CDs efficiently terminated the growth of amyloid fibrils. When we compared the amyloidogenicity of all four types of CNPs, the following trend was apparent: NDs > control > C60 > CDs > SWNTs. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2018
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