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Inflammation regulates 11 beta-hydroxysteroid dehydrogenase type 1 differentially in specific compartments of the gut mucosal immune system
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SYSNO ASEP 0479728 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Inflammation regulates 11 beta-hydroxysteroid dehydrogenase type 1 differentially in specific compartments of the gut mucosal immune system Tvůrce(i) Ergang, Peter (FGU-C) RID, ORCID
Vodička, Martin (FGU-C) RID, ORCID, SAI
Vagnerová, Karla (FGU-C) RID, ORCID
Moravec, Martin (FGU-C)
Kvapilová, Pavlína (FGU-C)
Kment, M. (CZ)
Pácha, Jiří (FGU-C) RID, ORCIDZdroj.dok. Steroids. - : Elsevier - ISSN 0039-128X
Roč. 126, Oct 2017 (2017), s. 66-73Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova metabolism of glucocorticoids ; cytokine microenvironment ; lymphoid organs ; colon ; Dextran sodium sulfate colitis Vědní obor RIV ED - Fyziologie Obor OECD Physiology (including cytology) CEP GAP303/10/0969 GA ČR - Grantová agentura ČR GA15-07268S GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000411539400009 EID SCOPUS 85028049692 DOI https://doi.org/10.1016/j.steroids.2017.07.007 Anotace The bioavailability of glucocorticoids is modulated by enzyme 11 beta-hydroxysteroid dehydrogenase type 1 (11HSD1), which catalyzes the conversion of inactive 11-oxo-glucocorticoids to active 11-hydroxy-glucocorticoids cortisol and corticosterone and is regulated by pro-inflammatory cytokines. Our aim was to assess the effect of colitis on the expression of 11HSD1 in specific microanatomical compartments of the mucosal immune system. Using qRT-PCR we quantified the expression of 11HSD1 and cytokines in the colon, mesenteric lymph nodes (MLN) and spleen of mice with colitis. Microsamples of the MLN cortex, paracortex and medulla, colonic crypt epithelium (CCE), lamina propria and isolated intestinal lymphoid follicles (ILF) were harvested by laser microdissection, whereas splenic and MLN lymphocytes by flow cytometry. Colitis increased 11HSD1 in the CCE, ILF, and MLN cortex but not in the lamina propria and the MLN paracortex and medulla. Expression of IL-4, IL-21 and TNFa was increased in both the cortex of MLN and ILF, whereas IL-1 beta and IL-10 were only increased in the follicles. No positive effect was observed in the case of IFN gamma and TGF beta. 11HSD1 was positively correlated with TNFa and less strongly with IL-21, IL-1 beta, and IL-4. Colitis also upregulated the 11HSD1 expression of T cells in the spleen and MLN. The study demonstrates the stimulatory effect of inflammation on local glucocorticoid metabolism only in particular compartments of the mucosal immune system. The correlation between cytokines and 11HSD1 in the ILF and MLN cortex indicates that pro-inflammatory cytokines may amplify glucocorticoid signals in inductive compartments of the mucosal immune system. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2018
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