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Tick thioester-containing proteins and phagocytosis do not affect transmission of Borrelia afzelii from the competent vector ixodes ricinus
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SYSNO ASEP 0479564 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Tick thioester-containing proteins and phagocytosis do not affect transmission of Borrelia afzelii from the competent vector ixodes ricinus Tvůrce(i) Urbanová, Veronika (BC-A) RID, ORCID
Hajdušek, Ondřej (BC-A) RID, ORCID
Hönig Mondeková, Helena (BC-A)
Šíma, Radek (BC-A) RID, ORCID
Kopáček, Petr (BC-A) RID, ORCIDCelkový počet autorů 5 Číslo článku 73 Zdroj.dok. Frontiers in Cellular and Infection Microbiology. - : Frontiers Media - ISSN 2235-2988
Roč. 7, MAR (2017)Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Borrelia ; complement ; Ixodes ; phagocytosis ; thioester-containing proteins Vědní obor RIV EC - Imunologie Obor OECD Immunology CEP GJ15-12006Y GA ČR - Grantová agentura ČR GA13-11043S GA ČR - Grantová agentura ČR GA17-27386S GA ČR - Grantová agentura ČR GA17-27393S GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000396351500001 EID SCOPUS 85021272739 DOI 10.3389/fcimb.2017.00073 Anotace The present concept of the transmission of Lyme disease from Borrelia-infected Ixodes sp. ticks to the naïve host assumes that a low number of spirochetes that manage to penetrate the midgut epithelium migrate through the hemocoel to the salivary glands and subsequently infect the host with the aid of immunomodulatory compounds present in tick saliva. Therefore, humoral and/or cellular immune reactions within the tick hemocoel may play an important role in tick competence to act as a vector for borreliosis. To test this hypothesis we have examined complement-like reactions in the hemolymph of the hard tick Ixodes ricinus against Borrelia afzelii (the most common vector and causative agent of Lyme disease in Europe). We demonstrate that I. ricinus hemolymph does not exhibit borreliacidal effects comparable to complement-mediated lysis of bovine sera. However, after injection of B. afzelii into the tick hemocoel, the spirochetes were efficiently phagocytosed by tick hemocytes and this cellular defense was completely eliminated by pre-injection of latex beads. As tick thioester-containing proteins (T-TEPs) are components of the tick complement system, we performed RNAi-mediated silencing of all nine genes encoding individual T-TEPs followed by in vitro phagocytosis assays. Silencing of two molecules related to the C3 complement component (IrC3-2 and IrC3-3) significantly suppressed phagocytosis of B. afzelii, while knockdown of IrTep (insect type TEP) led to its stimulation. However, RNAi-mediated silencing of T-TEPs or elimination of phagocytosis by injection of latex beads in B. afzelii-infected I. ricinus nymphs had no obvious impact on the transmission of spirochetes to naïve mice, as determined by B. afzelii infection of murine tissues following tick infestation. This result supports the concept that Borrelia spirochetes are capable of avoiding complement-related reactions within the hemocoel of ticks competent to transmit Lyme disease. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2018
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