Membrane Binding of Recoverin: From Mechanistic Understanding to Biological Functionality

0478298 - ÚOCHB 2018 RIV US eng J - Článek v odborném periodiku
Timr, Štěpán - Pleskot, Roman - Kadlec, Jan - Kohagen, Miriam - Magarkar, Aniket - Jungwirth, Pavel
Membrane Binding of Recoverin: From Mechanistic Understanding to Biological Functionality.
ACS Central Science. Roč. 3, č. 8 (2017), s. 868-874. ISSN 2374-7943. E-ISSN 2374-7951
Grant CEP: GA ČR(CZ) GBP208/12/G016
Institucionální podpora: RVO:61388963
Klíčová slova: recoverin * membrane * myristoyl * molecular dynamics
Obor OECD: Physical chemistry
Impakt faktor: 11.228, rok: 2017
http://pubs.acs.org/doi/full/10.1021/acscentsci.7b00210

Recoverin is a neuronal calcium sensor involved in vision adaptation that reversibly associates with cellular membranes via its calcium-activated myristoyl switch. While experimental evidence shows that the myristoyl group significantly enhances membrane affinity of this protein, molecular details of the binding process are still under debate. Here, we present results of extensive molecular dynamics simulations of recoverin in the proximity of a phospholipid bilayer. We capture multiple events of spontaneous membrane insertion of the myristoyl moiety and confirm its critical role in the membrane binding. Moreover, we observe that the binding strongly depends on the conformation of the N-terminal domain. We propose that a suitable conformation of the N-terminal domain can be stabilized by the disordered C-terminal segment or by binding of the target enzyme, i.e., rhodopsin kinase. Finally, we find that the presence of negatively charged lipids in the bilayer stabilizes a physiologically functional orientation of the membrane-bound recoverin.
Trvalý link: http://hdl.handle.net/11104/0274407