0476489 - ÚEB 2018 RIV US eng J - Článek v odborném periodiku
Moyo, M. - Aremu, A.O. - Chukwujekwu, J. C. - Grúz, Jiří - Skořepa, Jiří - Doležal, Karel - Katsvanga, C. A. T. - Van Staden, J.
Phytochemical Characterization, Antibacterial, Acetylcholinesterase Inhibitory and Cytotoxic Properties of Cryptostephanus vansonii, an Endemic Amaryllid.
Phytotherapy Research. Roč. 31, č. 5 (2017), s. 713-720. ISSN 0951-418X. E-ISSN 1099-1573
Grant CEP: GA MŠMT(CZ) LO1204; GA MŠMT LK21306
Institucionální podpora: RVO:61389030
Klíčová slova: in-vitro * alzheimers-disease * alkaloids * extracts * antioxidant * coumarins * apoptosis * phylogeny * medicine * bacteria * acetylcholinesterase inhibition * Amaryllidaceae * antibacterial * cytotoxicity * flavonoids * phenolic acids
Obor OECD: Plant sciences, botany
Impakt faktor: 3.349, rok: 2017 ; AIS: 0.548, rok: 2017
DOI: https://doi.org/10.1002/ptr.5788

Cryptostephanus vansonii I. Verd., an endemic Amaryllidaceae species from Zimbabwe, was evaluated for its acetylcholinesterase (AChE) inhibitory and cytotoxicity properties using Ellman's colorimetric method and the tetrazolium-based colorimetric assay against Vero monkey kidney cells, respectively. The plant extracts were also evaluated for their antibacterial activity against five bacteria. Furthermore, phytochemical profiles of the extracts were determined using ultra-high performance liquid chromatography coupled with tandem mass spectrometry analysis. A plant part-dependent AChE inhibitory activity was observed, in the order, root>rhizome>basal leaf>leaf. Overall, C. vansonii extracts exhibited better antibacterial activity against Gram-negative compared with Gram-positive bacteria. Cytotoxic effects were not detected in Vero monkey kidney cell lines suggesting the possible absence of toxophores in C. vansonii extracts. Similar to the trend in biological activity, a distinct plant part-dependent variation in hydroxybenzoates, hydroxycinnamates and flavonoids was observed in the plant extracts. In addition, 5-hydroxymetylfurfural and eucomic acid were detected in the different plant parts of C. vansonii. The results of the present study provide valuable AChE inhibition activity, toxicological and phytochemical profiles of C. vansonii. Further studies on isolation of bioactive compounds and their subsequent evaluation in other pharmacological and toxicological model systems are required.
Trvalý link: http://hdl.handle.net/11104/0272977