Počet záznamů: 1  

Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered

  1. 1.
    SYSNO ASEP0476382
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevLipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
    Tvůrce(i) Koukalová, Alena (UFCH-W) RID
    Amaro, Mariana (UFCH-W) RID, ORCID
    Aydogan, Gokcan (UFCH-W)
    Gröbner, G. (SE)
    Williamson, P. T. F. (GB)
    Mikhalyov, I. (RU)
    Hof, Martin (UFCH-W) RID, ORCID
    Šachl, Radek (UFCH-W) RID, ORCID
    Číslo článku5460
    Zdroj.dok.Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
    Roč. 7, JUL 2017 (2017)
    Poč.str.12 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovaFLUORESCENCE CORRELATION SPECTROSCOPY ; PLASMA-MEMBRANE VESICLES ; RESONANCE ENERGY-TRANSFER
    Vědní obor RIVCF - Fyzikální chemie a teoretická chemie
    Obor OECDPhysical chemistry
    CEPGA17-03160S GA ČR - Grantová agentura ČR
    Způsob publikováníOpen access
    Institucionální podporaUFCH-W - RVO:61388955
    UT WOS000405464200097
    EID SCOPUS85024403161
    DOI10.1038/s41598-017-05539-y
    AnotaceIt is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesterol
    (Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on model
    membranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although the
    existence of such microdomains has not been proven for the plasma membrane, such lipid mixtures
    have been often used as a model system for ‘rafts’. On the other hand, recent super resolution and
    single molecule results indicate that the plasma membrane might organize into nanocompartments.
    However, due to the limited resolution of those techniques their unambiguous characterization
    is still missing. In this work, a novel combination of Förster resonance energy transfer and Monte
    Carlo simulations (MC-FRET) identifies directly 10 nm large nanodomains in liquid-disordered model
    membranes composed of lipid mixtures containing SM and Chol. Combining MC-FRET with solidstate
    wide-line and high resolution magic angle spinning NMR as well as with fluorescence correlation
    spectroscopy we demonstrate that these nanodomains containing hundreds of lipid molecules are fluid
    and disordered. In terms of their size, fluidity, order and lifetime these nanodomains may represent a
    relevant model system for cellular membranes and are closely related to nanocompartments suggested
    to exist in cellular membranes.
    PracovištěÚstav fyzikální chemie J.Heyrovského
    KontaktMichaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
    Rok sběru2018
Počet záznamů: 1  

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