Počet záznamů: 1
Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
- 1.
SYSNO ASEP 0476382 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered Tvůrce(i) Koukalová, Alena (UFCH-W) RID
Amaro, Mariana (UFCH-W) RID, ORCID
Aydogan, Gokcan (UFCH-W)
Gröbner, G. (SE)
Williamson, P. T. F. (GB)
Mikhalyov, I. (RU)
Hof, Martin (UFCH-W) RID, ORCID
Šachl, Radek (UFCH-W) RID, ORCIDČíslo článku 5460 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 7, JUL 2017 (2017)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova FLUORESCENCE CORRELATION SPECTROSCOPY ; PLASMA-MEMBRANE VESICLES ; RESONANCE ENERGY-TRANSFER Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Obor OECD Physical chemistry CEP GA17-03160S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000405464200097 EID SCOPUS 85024403161 DOI 10.1038/s41598-017-05539-y Anotace It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesterol
(Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on model
membranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although the
existence of such microdomains has not been proven for the plasma membrane, such lipid mixtures
have been often used as a model system for ‘rafts’. On the other hand, recent super resolution and
single molecule results indicate that the plasma membrane might organize into nanocompartments.
However, due to the limited resolution of those techniques their unambiguous characterization
is still missing. In this work, a novel combination of Förster resonance energy transfer and Monte
Carlo simulations (MC-FRET) identifies directly 10 nm large nanodomains in liquid-disordered model
membranes composed of lipid mixtures containing SM and Chol. Combining MC-FRET with solidstate
wide-line and high resolution magic angle spinning NMR as well as with fluorescence correlation
spectroscopy we demonstrate that these nanodomains containing hundreds of lipid molecules are fluid
and disordered. In terms of their size, fluidity, order and lifetime these nanodomains may represent a
relevant model system for cellular membranes and are closely related to nanocompartments suggested
to exist in cellular membranes.Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2018
Počet záznamů: 1