Počet záznamů: 1
The use of histological methods to detect testicular pathology.\n\n
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SYSNO ASEP 0474840 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název The use of histological methods to detect testicular pathology.
Tvůrce(i) Margaryan, Hasmik (BTO-N) RID
Pavlínková, Gabriela (BTO-N) RID, ORCID
Bohuslavová, Romana (BTO-N) RID
Dvořáková-Hortová, Kateřina (BTO-N)
Pěknicová, Jana (BTO-N) RIDCelkový počet autorů 5 Zdroj.dok. Book of abstracts XXIIIrd Symposium of Immunology and Biology of Reproduction. - Vestec u Prahy : Biotechnologický ústav AVČR v. v. i., 2017 / Kubátová alena
S. 21-22Poč.str. 2 s. Akce XXIIIrd Symposium of Immunology and Biology of Reproduction Datum konání 18.05.2017 - 20.05.2017 Místo konání Třešť Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova diabetes mellitus ; monoclonal antibody ; spermatogenesis ; mitosis ; meiosis ; seminiferous tubules Vědní obor RIV EC - Imunologie Obor OECD Biochemistry and molecular biology CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy AZV 15-30880A GA MZd - Ministerstvo zdravotnictví Institucionální podpora BTO-N - RVO:86652036 Anotace Histological analysis of the testes may help to determine the scope of tissue damage under the influence of various substances (endocrine disruptors) as well as metabolic diseases (diabetes mellitus). Using hematoxylin & eosin stained sections, the morphometric evaluations of seminiferous tubules can be performed, particularly the tubule diameter and thickness of the seminiferous epithelium.
In our case, we used a panel of antibodies and mouse model to evaluate changes in the testicular tissue affected by metabolic disease - diabetes mellitus.
Selected antibodies against different proteins were used for detection of germ cells during spermatogenesis, mitotic and meiotic division, tissue architecture and other parameters.
The histology of control testes showed smooth rounded seminiferous tubules flanked by embryonic epithelium at various stages of spermatogenesis. In contrast, in the testes of diabetic mice, thinner disrupted germinal epithelium was noticeable, although all the distinct developmental stages of spermatogenesis, including spermatozoa in the lumen of the seminiferous tubules were identified. We observed high individual variations in testicular histology within the diabetic group. Some seminiferous tubules showed the early spermatocytes and spermatids, which did not form regular "columns". Premature spermatocytes or some seminiferous tubules were anthropic without any cells in the tubule lumen.
The panel of antibodies can be used to test differences in healthy and damaged organs in other mammals, including human.
Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2018
Počet záznamů: 1