Počet záznamů: 1
Cytotoxicity of poly(p-phenylenediamine)
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SYSNO ASEP 0472634 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cytotoxicity of poly(p-phenylenediamine) Tvůrce(i) Kuceková, Z. (CZ)
Rejmontová, P. (CZ)
Humpolíček, P. (CZ)
Kašpárková, V. (CZ)
Bober, Patrycja (UMCH-V) RID, ORCID, SAI
Sáha, P. (CZ)
Stejskal, Jaroslav (UMCH-V) RID, ORCID, SAIZdroj.dok. Chemical Papers. - : Springer - ISSN 0366-6352
Roč. 71, č. 2 (2017), s. 367-372Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. SK - Slovensko Klíč. slova cytotoxicity ; poly(p-phenylenediamine) ; mouse embryonic fibroblasts Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science CEP GA13-00270S GA ČR - Grantová agentura ČR Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000394523000013 EID SCOPUS 85003952274 DOI https://doi.org/10.1007/s11696-016-0069-4 Anotace Although poly(p-phenylenediamine) is an electric non-conductor, it exhibits, analogously to conducting polymers, redox activity and could, therefore, find applications in biomedicine. In the current work, the cytotoxicity of poly(p-phenylenediamine) polymer powder produced by the chemical oxidation of p-phenylenediamine with ammonium peroxydisulfate in acidic aqueous media has been studied. Primary mouse embryonic fibroblasts were used for this purpose. Interestingly, the standard methods for the determination of polymer cytotoxicity based on international standard EN ISO 10993-5 could not be applied. The reason was the interaction of polymer extracts with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. On the basis of the evaluation of flow cytometry and micrographs taken by fluorescence microscopy on cells treated with extracts of poly(p-phenylenediamine), it can be concluded that the powder polymer possesses severe cytotoxicity. The results suggest that practical application of the polymer within biomedicine is, at the current state of knowledge, difficult, and modification of the preparation techniques and/or subsequent purification of poly(p-phenylenediamine) is needed. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2017
Počet záznamů: 1