0469461 - FGÚ 2017 RIV US eng J - Článek v odborném periodiku
Kraidlová, Lucie - Schrevens, S. - Tournu, H. - Van Zeebroeck, G. - Sychrová, Hana - Van Dijck, P.
Characterization of the Candida albicans Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an S-Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis.
mSphere. Roč. 1, č. 6 (2016), č. článku e00284-16. ISSN 2379-5042. E-ISSN 2379-5042
Grant CEP: GA ČR(CZ) GA16-03398S
Institucionální podpora: RVO:67985823
Klíčová slova: Candida albicans * GAP1 * S-adenosyl methionine * general amino acid permease * morphogenesis
Kód oboru RIV: EE - Mikrobiologie, virologie

Amino acids are key sources of nitrogen for growth of Candida albicans. In order to detect and take up these amino acids from a broad range of different and changing nitrogen sources inside the host, this fungus must be able to adapt via its expression of genes for amino acid uptake and further metabolism. We analyzed six C. albicans putative general amino acid permeases based on their homology to the Saccharomyces cerevisiae Gap1 general amino acid permease. We generated single- and multiple-deletion strains and found that, based on growth assays and transcriptional or posttranscriptional regulation, Gap2 is the functional orthologue to ScGap1, with broad substrate specificity. Expression analysis showed that expression of all GAP genes is under control of the Csy1 amino acid sensor, which is different from the situation in S. cerevisiae. We show that Gap4 is the functional orthologue of ScSam3, the only S-adenosylmethionine (SAM) transporter in S. cerevisiae, and we report that Gap4 is required for SAM-induced morphogenesis.
Trvalý link: http://hdl.handle.net/11104/0267256