Single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells

0468793 - ÚIACH 2017 GB eng A - Abstrakt
Klepárník, Karel - Ledvina, Vojtěch - Janečková, Eva - Matalová, Eva
Single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells.
Congress Workbook. London, 2016.
[Single Cell Analysis Congress /4./. 10.11.2016-11.11.2016, London]
Grant CEP: GA ČR(CZ) GA14-28254S
Institucionální podpora: RVO:68081715 ; RVO:67985904
Klíčová slova: bioluminescence * single-cell analysis * caspase-3/7
Kód oboru RIV: CB - Analytická chemie, separace

Bioluminescent assay based on modified aminoluciferin was used for determination of apoptotic proteases caspase-3/7. A limit of detection beyond the average content in single cells was reached by using a photomultiplier with cooled photocathode working in photon counting regime and by miniaturization of the detection cell volume to 4 μl.
Trvalý link: http://hdl.handle.net/11104/0266618