Počet záznamů: 1
Proteomic Profiling and Analytical Chemistry: The Crossroads
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SYSNO ASEP 0467977 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Protein Extraction and Precipitation Tvůrce(i) Novák, Petr (MBU-M) RID, ORCID
Havlíček, Vladimír (MBU-M) RID, ORCIDZdroj.dok. Proteomic Profiling and Analytical Chemistry: The Crossroads. - Amsterdam : Elsevier, 2016 - ISBN 978-044463688-1 Rozsah stran s. 52-62 Poč.str. 11 s. Poč.str.knihy 282 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova analytical chemists ; mass spectrometrists ; researchers in proteomics Vědní obor RIV EE - Mikrobiologie, virologie CEP LO1509 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 EID SCOPUS 84969622242 DOI https://doi.org/10.1016/B978-0-444-63688-1.00004-5 Anotace Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure Includes practical examples illustrating the importance of problems in quantitation and validation of biomarkers Helps in designing and executing proteomic experiments with sound analytics. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
Počet záznamů: 1