Počet záznamů: 1
Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria
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SYSNO ASEP 0467724 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria Tvůrce(i) Schulz, F. (AT)
Tyml, Tomáš (BC-A)
Pizzetti, I. (IT)
Dyková, I. (CZ)
Fazi, S. (IT)
Kostka, Martin (BC-A) RID
Horn, M. (AT)Celkový počet autorů 7 Číslo článku 13381 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 5, 25 August (2015)Poč.str. 10 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova free-living amebas ; bacterial endosymbionts ; phylogenetic inference ; Paramecium caudatum ; Naegleria ; gene expression ; environment ; chlamydiae ; diversity ; pathogens Vědní obor RIV EG - Zoologie CEP GBP505/12/G112 GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000360035600002 EID SCOPUS 84940056585 DOI https://doi.org/10.1038/srep13381 Anotace Amoebae play an important ecological role as predators in microbial communities. They also serve as niche for bacterial replication, harbor endosymbiotic bacteria and have contributed to the evolution of major human pathogens. Despite their high diversity, marine amoebae and their association with bacteria are poorly understood. Here we describe the isolation and characterization of two novel marine amoebae together with their bacterial endosymbionts, tentatively named 'Candidatus Occultobacter vannellae' and 'Candidatus Nucleophilum amoebae'. While one amoeba strain is related to Vannella, a genus common in marine habitats, the other represents a novel lineage in the Amoebozoa. The endosymbionts showed only low similarity to known bacteria (85-88% 16S rRNA sequence similarity) but together with other uncultured marine bacteria form a sister clade to the Coxiellaceae. Using fluorescence in situ hybridization and transmission electron microscopy, identity and intracellular location of both symbionts were confirmed; one was replicating in host-derived vacuoles, whereas the other was located in the perinuclear space of its amoeba host. This study sheds for the first time light on a so far neglected group of protists and their bacterial symbionts. The newly isolated strains represent easily maintainable model systems and pave the way for further studies on marine associations between amoebae and bacterial symbionts. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2017
Počet záznamů: 1