Počet záznamů: 1  

Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species

  1. 1.
    SYSNO ASEP0466486
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevOxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species
    Tvůrce(i) Wilhelm, Jiří (FGU-C) RID
    Vytášek, Richard (FGU-C)
    Uhlík, Jiří (FGU-C)
    Vajner, Luděk (FGU-C)
    Číslo článku5057610
    Zdroj.dok.Oxidative Medicine and Cellular Longevity. - : Hindawi - ISSN 1942-0900
    Roč. 2016, č. 2016 (2016)
    Poč.str.12 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaoxidative stress ; developing rat brain ; lipid peroxidation
    Vědní obor RIVED - Fyziologie
    CEPGAP303/11/0298 GA ČR - Grantová agentura ČR
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000377047800001
    EID SCOPUS84966335514
    DOI https://doi.org/10.1155/2016/5057610
    AnotaceOxidative stress after birth led us to localize reactive oxygen and nitrogen species (RONS) production in the developing rat brain. Brains were assessed a day prenatally and on postnatal days 1, 2, 4, 8, 14, 30, and 60. Oxidation of dihydroethidium detected superoxide; 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate revealed hydrogen peroxide; immunohistochemical proof of nitrotyrosine and carboxyethyllysine detected peroxynitrite formation and lipid peroxidation, respectively. Blue autofluorescence detected protein oxidation. The foetuses showed moderate RONS production, which changed cyclically during further development. The periods and sites of peak production of individual RONS differed, suggesting independent generation. On day 1, neuronal/glial RONS production decreased indicating that increased oxygen concentration after birth did not cause oxidative stress. Dramatic changes in the amount and the sites of RONS production occurred on day 4. Nitrotyrosine detection reached its maximum. Day 14 represented other vast alterations in RONS generation. Superoxide production in arachnoidal membrane reached its peak. From this day on, the internal elastic laminae of blood vessels revealed the blue autofluorescence. The adult animals produced moderate levels of superoxide; all other markers reached their minimum. There was a strong correlation between detection of nitrotyrosine and carboxyethyllysine probably caused by lipid peroxidation initiated with RONS.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2017
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.