Počet záznamů: 1
Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species
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SYSNO ASEP 0466486 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species Tvůrce(i) Wilhelm, Jiří (FGU-C) RID
Vytášek, Richard (FGU-C)
Uhlík, Jiří (FGU-C)
Vajner, Luděk (FGU-C)Číslo článku 5057610 Zdroj.dok. Oxidative Medicine and Cellular Longevity. - : Hindawi - ISSN 1942-0900
Roč. 2016, č. 2016 (2016)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova oxidative stress ; developing rat brain ; lipid peroxidation Vědní obor RIV ED - Fyziologie CEP GAP303/11/0298 GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000377047800001 EID SCOPUS 84966335514 DOI https://doi.org/10.1155/2016/5057610 Anotace Oxidative stress after birth led us to localize reactive oxygen and nitrogen species (RONS) production in the developing rat brain. Brains were assessed a day prenatally and on postnatal days 1, 2, 4, 8, 14, 30, and 60. Oxidation of dihydroethidium detected superoxide; 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate revealed hydrogen peroxide; immunohistochemical proof of nitrotyrosine and carboxyethyllysine detected peroxynitrite formation and lipid peroxidation, respectively. Blue autofluorescence detected protein oxidation. The foetuses showed moderate RONS production, which changed cyclically during further development. The periods and sites of peak production of individual RONS differed, suggesting independent generation. On day 1, neuronal/glial RONS production decreased indicating that increased oxygen concentration after birth did not cause oxidative stress. Dramatic changes in the amount and the sites of RONS production occurred on day 4. Nitrotyrosine detection reached its maximum. Day 14 represented other vast alterations in RONS generation. Superoxide production in arachnoidal membrane reached its peak. From this day on, the internal elastic laminae of blood vessels revealed the blue autofluorescence. The adult animals produced moderate levels of superoxide; all other markers reached their minimum. There was a strong correlation between detection of nitrotyrosine and carboxyethyllysine probably caused by lipid peroxidation initiated with RONS. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2017
Počet záznamů: 1