Počet záznamů: 1
Centromeric and non-centromeric satellite DNA organisation differs in holocentric Rhynchospora species
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SYSNO ASEP 0464891 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Centromeric and non-centromeric satellite DNA organisation differs in holocentric Rhynchospora species Tvůrce(i) Ribeiro, T. (BR)
Marques, A. (BR)
Novák, Petr (BC-A) RID, ORCID
Schubert, V. (DE)
Vanzela, A.L.L. (BR)
Macas, Jiří (BC-A) RID, ORCID, SAI
Houben, A. (DE)
Pedrosa-Harand, A. (BR)Celkový počet autorů 8 Zdroj.dok. Chromosoma. - : Springer - ISSN 0009-5915
Roč. 126, September 19 (2017), s. 325-335Poč.str. 11 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Chromocentre ; Cyperaceae ; Heterochromatin ; Holocentric chromosome Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP GBP501/12/G090 GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000397951000010 EID SCOPUS 84988431922 DOI 10.1007/s00412-016-0616-3 Anotace Satellite DNA repeats (or satDNA) are fast-evolving sequences usually associated with condensed heterochromatin. To test whether the chromosomal organisation of centromeric and non-centromeric satDNA differs in species with holocentric chromosomes, we identified and characterised the major satDNA families in the holocentric Cyperaceae species Rhynchospora ciliata (2n = 10), R. globosa (2n = 50) and R. tenuis (2n = 2x = 4 and 2n = 4x = 8). While conserved centromeric repeats (present in R. ciliata and R. tenuis) revealed linear signals at both chromatids, non-centromeric, species-specific satDNAs formed distinct clusters along the chromosomes. Colocalisation of both repeat types resulted in a ladder-like hybridisation pattern at mitotic chromosomes. In interphase, the centromeric satDNA was dispersed while non-centromeric satDNA clustered and partly colocalised to chromocentres. Despite the banding-like hybridisation patterns of the clustered satDNA, the identification of chromosome pairs was impaired due to the irregular hybridisation patterns of the homologues in R. tenuis and R. ciliata. These differences are probably caused by restricted or impaired meiotic recombination as reported for R. tenuis, or alternatively by complex chromosome rearrangements or unequal condensation of homologous metaphase chromosomes. Thus, holocentricity influences the chromosomal organisation leading to differences in the distribution patterns and condensation dynamics of centromeric and non-centromeric satDNA. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2018
Počet záznamů: 1