Počet záznamů: 1
Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16
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SYSNO ASEP 0464550 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16 Tvůrce(i) Samek, Ota (UPT-D) RID, ORCID, SAI
Obruča, S. (CZ)
Šiler, Martin (UPT-D) RID, ORCID, SAI
Sedláček, P. (CZ)
Benešová, P. (CZ)
Kučera, D. (CZ)
Márová, I. (CZ)
Ježek, Jan (UPT-D) RID, ORCID, SAI
Bernatová, Silvie (UPT-D) RID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDCelkový počet autorů 10 Zdroj.dok. Sensors. - : MDPI
Roč. 16, č. 11 (2016), 1808:1-7Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Raman spectroscopy ; Cupriavidus necator H16 ; polyhydroxyalkanoates Vědní obor RIV BH - Optika, masery a lasery CEP GA15-20645S GA ČR - Grantová agentura ČR LO1212 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED0017/01/01 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UPT-D - RVO:68081731 UT WOS 000389641700041 EID SCOPUS 84994791887 DOI 10.3390/s16111808 Anotace We report herein on the application of Raman spectroscopy to the rapid quantitative analysis of polyhydroxyalkanoates (PHAs), biodegradable polyesters accumulated by various bacteria. This theme was exemplified for quantitative detection of the most common member of PHAs, poly(3-hydroxybutyrate) (PHB) in Cupriavidus necator H16. We have identified the relevant spectral region (800–1800 cm(−1)) incorporating the Raman emission lines exploited for the calibration of PHB (PHB line at 1736 cm(−1)) and for the selection of the two internal standards (DNA at 786 cm(−1)) and Amide I at 1662 cm(−1)). In order to obtain quantitative data for calibration of intracellular content of PHB in bacterial cells reference samples containing PHB amounts—determined by gas chromatography—from 12 percent to 90 percent (w/w) were used. Consequently, analytical results based on this calibration can be used for fast and reliable determination of intracellular PHB content during biotechnological production of PHB since the whole procedure—from bacteria sampling, centrifugation, and sample preparation to Raman analysis—can take about 12 min. In contrast, gas chromatography analysis takes approximately 8 h. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2017
Počet záznamů: 1