Analysis of mice organs and blood from inhalation experiments with selected metal oxides nanoparticles

0464273 - ÚIACH 2017 SK eng A - Abstrakt
Dočekal, Bohumil - Večeřa, Zbyněk - Mikuška, Pavel - Šurýn, P. - Dufka, Michaela - Coufalík, Pavel
Analysis of mice organs and blood from inhalation experiments with selected metal oxides nanoparticles.
XXIst Slovak-Czech Spectroscopic Conference. Book of abstracts. Košice: Technická univerzita v Košiciach, 2016 - (Remeteiová, D.; Ružičková, S.). s. 23-23. ISBN 978-80-553-2636-8.
[Slovak-Czech Spectroscopic Conference /21./. 16.10.2016-20.10.2016, Liptovský Ján,]
Grant CEP: GA ČR(CZ) GAP503/11/2315; GA ČR(CZ) GBP503/12/G147
Institucionální podpora: RVO:68081715
Klíčová slova: mice organs * electrothermal atomic absorption spectrometry * inductively coupled plasma mass spectrometry * inhaled nanoparticles
Kód oboru RIV: CB - Analytická chemie, separace

This contribution focusses on the study of fate of inhaled nanoparticles of Cd, Mn, Pb, Ti and Zn oxides in the organism of small “white” mice (female, ICR strain), on translocation of metals from primary to secondary target organs by blood. Some critical steps of trace and ultra-trace determination in analysis of individual organs (lung, liver, kidney, spleen, heart and brain) and blood fractions (blood cells, protein and low molecular fractions) are shown. Methods of taking samples, sample preparation and decomposition by various procedures (microwave assisted high pressure mineralization, dry ashing in nitrous oxide/ozone atmosphere - APION) are presented. Some spectral interference effects in determination of selected metals by electrothermal atomic absorption spectrometry and inductively coupled plasma mass spectrometry are discussed. Basic characteristics of nanoparticles used and consequences of their inhalation to organ tissues and cellular structures are also given on transmission electron microscopy and histology pictures.
Trvalý link: http://hdl.handle.net/11104/0263221