Počet záznamů: 1
Study of sperm proteins in different mammalian species
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SYSNO ASEP 0463007 Druh ASEP K - Konferenční příspěvek (lokální konf.) Zařazení RIV Záznam nebyl označen do RIV Název Study of sperm proteins in different mammalian species Tvůrce(i) Pohlová, Alžběta (BTO-N)
Zigo, Michal (BTO-N) RID
Jonáková, Věra (BTO-N) RID
Postlerová, Pavla (BTO-N) ORCID, RIDCelkový počet autorů 4 Zdroj.dok. Book of abstracts of XXIInd Symposium of biology and immunology of reproduction. - Vestec u Prahy : Biotechnologický ústav AVČR, 2016 / Pěknicová Jana ; Elzeinová Fatima ; Kubátová Alena
S. 29-29Poč.str. 1 s. Akce XXIInd Symposium of biology and immunology of reproduction with international participation Datum konání 26.05.2016-28.05.2016 Místo konání Třešť Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Klíč. slova sperm surface proteins ; glycoproteins profiles ; sperm - zona pellucida interaction ; monoclonal antibodies Vědní obor RIV CE - Biochemie CEP GA 14-05547S GA ČR - Grantová agentura ČR Institucionální podpora BTO-N - RVO:86652036 Anotace Reproduction is an essential feature of all animals and a fundamental step to produce new generations. Study of sperm proteins is crucial for understanding of the sperm-egg recognition. We searched out sperm surface proteins involving in the zona pellucida (ZP) binding and studied whether these proteins are preserved throughout mammalian species. Indirect immunofluorescent technique was used to test a panel of monoclonal antibodies prepared against boar sperm surface proteins on spermatozoa of bull and mice. We found a cross-reactivity of some antibodies against boar sperm with bull ejaculated and mouse epididymal spermatozoa. Further, we isolated sperm proteins from different mammalian species, such as pig, bull, dog, cat, mouse and human. Proteins were separated by SDS-electrophoresis and protein/glycoprotein profiles from epididymal, ejaculated and in vitro capacitated sperm were compared. The interaction of sperm with ZP was studied on electrophoretically-separated sperm surface proteins from pig and bull with biotin-labeled ZP glycoproteins using Far Western blot. Antibodies, which reacted with boar sperm surface proteins with ZP-binding activity, therefore could be potential egg-binding receptors, were used for monitoring of the sperm protein origin in reproductive fluids and tissues. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2017
Počet záznamů: 1