Počet záznamů: 1
Possible role of spermatogenic protein glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) in mammalian sperm
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SYSNO ASEP 0462995 Druh ASEP K - Konferenční příspěvek (lokální konf.) Zařazení RIV Záznam nebyl označen do RIV Název Possible role of spermatogenic protein glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) in mammalian sperm Tvůrce(i) Margaryan, Hasmik (BTO-N) RID
Dorosh, Andriy (BTO-N) RID
Čapková, Jana (BTO-N)
Postlerová, Pavla (BTO-N) ORCID, RID
Philimonenko, Anatoly (UMG-J)
Hozák, Pavel (UMG-J) RID, ORCID
Pěknicová, Jana (BTO-N) RIDCelkový počet autorů 7 Zdroj.dok. Book of abstracts of XXIst Symposium of biology and immunology of reproduction. - Praha : Biotechnologický ústav AVČR, 2015 / Pěknicová Jana ; Elzeinová Fatima ; Kubátová Alena
S. 31-31Poč.str. 1 s. Akce XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION Datum konání 14.05.2015-16.05.2015 Místo konání Třešť Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova GAPDHS ; sperm glycolytic enzyme ; zona pellucida glycoprotein matrix ; monoclonal antibody Vědní obor RIV CE - Biochemie CEP GA P503/12/1834 GA ČR - Grantová agentura ČR Anotace Sperm proteins are important for the structure and function of these specific, highly differentiated cells. Certain of these proteins play a role in sperm-egg recognition during primary or secondary binding at zona pellucida glycoprotein matrix. The aim of this study was to characterize the acrosomal sperm protein recognized by a monoclonal antibody (MoAb) Hs-8, prepared in our laboratory by immunization of BALB/c mice with human ejaculated sperm, and to test the possible role of this protein in gamete interaction. MoAb Hs-8 specifically labelled a 45 kDa protein from the sperm extract in the immunoblotting test. Sequence analysis identified this Hs-8 protein as GAPDHS. In order to perform a control tests, a commercial mouse anti-GAPDHS MoAb was applied. Both antibodies showed similar staining patterns using immunofluorescence labelling, transmission electron microscopy and immunoblot analysis. Moreover, both Hs-8 and commercial anti-GAPDHS antibodies blocked the secondary sperm-zona pellucida binding. Generally, GAPDHS was considered mainly as sperm-specific glycolytic enzyme involved in energy production during spermatogenesis and sperm motility and its role in the sperm head was unknown. In this study, we confirmed the potential additional role of GAPDHS as a binding protein that is involved in the sperm-zona pellucida interaction. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2017
Počet záznamů: 1