Počet záznamů: 1
Validate or falsify: Lessons learned from a microscopy method claimed to be useful for detecting Borrelia and Babesia organisms in human blood
- 1.
SYSNO ASEP 0461824 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Validate or falsify: Lessons learned from a microscopy method claimed to be useful for detecting Borrelia and Babesia organisms in human blood Tvůrce(i) Aase, A. (NO)
Hajdušek, Ondřej (BC-A) RID, ORCID
Øines, Ø. (NO)
Quarsten, H. (NO)
Wilhelmsson, P. (SE)
Herstad, T.K. (NO)
Kjelland, V. (NO)
Šíma, Radek (BC-A) RID, ORCID
Jalovecká, Marie (BC-A) RID
Lindgren, P-E. (SE)
Aaberge, I.S. (NO)Zdroj.dok. Infectious Diseases - ISSN 2374-4235
Roč. 48, č. 6 (2016), s. 411-419Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Lyme disease ; Borrelia burgdorferi sensu lato ; babesiosis ; Babesia spp. ; Lyme borreliosis ; PCR ; microscopy Vědní obor RIV EC - Imunologie CEP GP13-27630P GA ČR - Grantová agentura ČR GP13-12816P GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000373810800001 EID SCOPUS 84968662284 DOI 10.3109/23744235.2016.1144931 Anotace Background A modified microscopy protocol (the LM-method) was used to demonstrate what was interpreted as Borrelia spirochetes and later also Babesia sp., in peripheral blood from patients. The method gained much publicity, but was not validated prior to publication, which became the purpose of this study using appropriate scientific methodology, including a control group. Methods Blood from 21 patients previously interpreted as positive for Borrelia and/or Babesia infection by the LM-method and 41 healthy controls without known history of tick bite were collected, blinded and analysed for these pathogens by microscopy in two laboratories by the LM-method and conventional method, respectively, by PCR methods in five laboratories and by serology in one laboratory. Results Microscopy by the LM-method identified structures claimed to be Borrelia- and/or Babesia in 66% of the blood samples of the patient group and in 85% in the healthy control group. Microscopy by the conventional method for Babesia only did not identify Babesia in any samples. PCR analysis detected Borrelia DNA in one sample of the patient group and in eight samples of the control group; whereas Babesia DNA was not detected in any of the blood samples using molecular methods. Conclusions The structures interpreted as Borrelia and Babesia by the LM-method could not be verified by PCR. The method was, thus, falsified. This study underlines the importance of doing proper test validation before new or modified assays are introduced. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2017
Počet záznamů: 1